甲状旁腺相关蛋白对鼠胚髁突软骨细胞增殖和分化的调控  

Regulation of PTHrP in Proliferation and Differentiation of Chondrocytes of Condyle in Fetal Mouse

作　　者：刘来奎[1] 江宏兵[1] 殷新民[1] 李晓箐[2] 易新竹[2] 

机构地区：[1]南京医科大学口腔医学院口腔解剖生理教研室,江苏南京210029 [2]四川大学华西口腔医学院口腔解剖生理教研室,四川成都610041

出　　处：《华西口腔医学杂志》2006年第3期206-209,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30371555)

摘　　要：目的探讨甲状旁腺相关蛋白(PTHrP)对鼠胚髁突软骨细胞增殖和分化的影响。方法在体外分离培养鼠胚髁突软骨细胞的基础上,观察PTHrP对其软骨结节形成、碱性磷酸酶(ALP)活性等的影响。结果研究发现加入浓度分别为0.01nmol/L、0.1nmol/L、1nmol/L、10nmol/L的PTHrP后,0.01nmol/L组与对照组相比,形成的软骨结节数量在统计学上无显著性差异(P>0.05),而软骨结节数量从0.1nmol/L组开始与对照组相比明显增加,统计学上有显著性差异(P<0.05)。而ALP活性在0.1—10nmol/L组与对照组相比明显升高,统计学上有显著性差异(P<0.05)。阻断其受体后,实验组和对照组软骨结节形成的数目明显减少(P<0.05),而对其ALP活性的影响在实验组和对照组之间无显著性差异(P>0.05)。结论PTHrP通过其受体具有促进髁突软骨细胞增殖和分化的作用,其调控机制与其在生长板软骨细胞及下颌膜内成骨中的作用相似。Objective To investigate the regulatory mechanism of parathyroid hormone-related protein （PTHrP） in proliferation and differentiation of chondrocytes of condyle in fetal mouse. Methods Chondrocytes of condyle in fetal mouse were separated and cultured in vitro, the influence of PTHrP on proliferation and differentiation was observed. Results After two weeks＇ culture in 0.01 nmol/L, 0.1 nmol/L, 1 nmol/L, 10nmol/L human PTHrP, there was significant difference in the number of cartilage nodule formed between experiment group and control group （P〈0.05）, and there was no significant difference in 0.01 nmol/L group（P〉0.05）. Alkaline pbosphatase（ALP） activity was significantly intensified in experiment group and control group （P〈0.05）. Meanwhile, it was found that this function of promotion was lessened after anti-PTHR antibody used. Conclusion It can be seen that PTHrP, via its receptor, can promote proliferation and differentiation of chondrocytes of condyle, which resemble its modulation mechanism in epiphyseal growth plate cartilage intramembrane in mandibule.

关 键 词：甲状旁腺相关蛋白 甲状旁腺相关蛋白受体 髁突 软骨 

分 类 号：R780.2[医药卫生—口腔医学]