APO诱导6-OHDA所致PD模型大鼠的旋转行为及其形态学改变  

Apomorphine caused morphological changes in ventral tagmental area and abnormal rotations in rats with Parkinson disease induced by 6-hydroxydopamine

作　　者：徐蓉[1] 李光武[1] 姚玉芹[1] 

机构地区：[1]安徽医科大学神经生物学研究所,合肥230032

出　　处：《安徽医科大学学报》2006年第3期247-249,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然基金(编号:2003kj226)

摘　　要：目的探讨不同时间点阿朴吗啡(APO)腹腔注射6-OHDA所致帕金森病模型大鼠的行为学及中脑腹侧被盖区(VTA)形态学变化。方法6-OHDA单侧一点注射大鼠右侧SNc,特异性毁损DA能神经元;术后1、7、14、21d腹腔注射APO,观察旋转行为;利用Nissl染色、免疫组织化学ABC法,观察各时间点VTADA能神经元形态学变化和TH表达情况。结果APO诱发PD大鼠模型异常旋转行为,Nissl染色见PD大鼠左侧中脑VTA有神经细胞肿胀、坏死等变化,VTATH+神经元数量减少。结论APO能诱导6-OHDAPD模型大鼠的旋转行为,其强弱可能与TH+神经元数量直接相关。Objective To observe apomorphine （APO） caused morphological changes in ventral tagmental area （VTA） and abnormal rotations in rats with Parkinson disease induced by 6-hydroxydopamine. Methods 6-OHDA was injected into right SNc of rats. The abnormal rotations induced by APO were observed on 1,7,14 and 21 d. Nissl＇s body staining, and immunohistochemical avidin-biotinylated peroxidase complex method （ABC） were used to observe DA neuron changes in VTA and tyrosine hydroxylase （TH）expression. Results Apomorphine caused abnormal rotations, neuronal cellular swelling, necrosis alteration in the left mesencephalic VTA, and decreases in quantities of VTA TH positive neuron. Conclusion Apomorphine causes abnormal rotations in rats with Parkinson disease induced by 6-hydroxydopamine, and extent of the changes is correlated with quantity of TH positive neuron.

关 键 词：多巴胺 阿朴吗啡 腹侧被盖区 酪氨酸羟化酶 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R971.5[医药卫生—临床医学]