骨保护素基因修饰的大鼠骨髓基质细胞系的培养与鉴定  

The culture and identification of rat MSC cell lines modified by osteoprotegerin gene

作　　者：吴迪[1] 赵春晖[1] 梅陵宣[1] 

机构地区：[1]安徽医科大学口腔医学院,合肥230032

出　　处：《安徽医科大学学报》2006年第3期272-274,共3页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:C03031101);安徽省教育厅自然科学研究项目(编号:2004kj219);安徽医科大学基金(编号:2003zr01)

摘　　要：目的培养经骨保护素OPG修饰的大鼠骨髓基质细胞系并检测其表达能力,鉴定表达产物,为基因工程技术治疗牙周病提供物质基础。方法利用已构建的pSecTag-OPG真核分泌表达穿梭载体,用脂质体法将目的基因转染至大鼠骨髓基质细胞,应用免疫组织化学和Westernblot方法证实转染的细胞表达骨保护素。结果经免疫组织化学和Westernblot方法检测,在转染OPG的大鼠骨髓基质细胞中有OPG表达。结论本实验成功培养出分泌表达OPG的大鼠骨髓基质细胞系。Objective To study the ability of expression and secretion of eukaryotic expression plasmid of human osteoprotegenrin by detecting its expression in rat MSC cell lines so as to inform gene engineering therapy of periodontitis. Methods The constructed recombinant plasmid was transformed into rat MSC cell line and OPG expressed in rat MSC cell line was determined using immuinohistochemistry and western blot. Result Recombinant plasmid pSecTag-opg was transformed into rat MSC cell line, and OPG-expression cell line of rat MSC was selected and confirmed by immunohistochemistry and western blot. Conclusion OPG high-expression rat MSC cell line is determined.

关 键 词：成骨细胞 骨髓细胞 基因 克隆 分子 基因表达 

分 类 号：R394.3[医药卫生—医学遗传学] R392.11[医药卫生—基础医学]