降低PARP酶活性对培养细胞SCE及微核效应的影响  

EFFECTS OF LOWERING POLY (ADP-RIBOSE)POLYMERASE ACTIVITY ON SISTER CHROMATID EXCHANGES AND MICRONUCLEUS IN CULTURED CELLS

作　　者：杨其伟[1] 陈雅文[1] 宋智[1] 陈德风[1] 

机构地区：[1]天津师范大学生物系,南开大学分子生物学研究所

出　　处：《天津师大学报（自然科学版）》1996年第2期38-43,共6页

基　　金：国家自然科学基金

摘　　要：聚腺苷二磷酸核糖基化作用是细胞内的一种重要的核蛋白转译后加工修饰，它参与细胞内很多重要的生物事件．催化该反应的酶是聚腺苷二磷酸核糖合酶（ＰＡＲＰ），底物为ＮＡＤ．本文使用ＰＡＲＰ酶的抑制剂苯甲酰胺处理培养细胞，研究了降低ＰＡＲＰ酶活性对培养细胞姐妹染色单体交换及微核效应的影响，为全面评价ＰＡＲＰ酶抑制剂在细胞内的功效打下基础．Poly (ADP-ribose) ation is a unique post-translational modification for nuclear proteins that catalyzed by poly (ADP-ribose) polymerase using NAD as a substrate and it is involved in many biological events. In this paper, the effects of benzamide, an inhibitor of poly (ADP-ribose) polymerase, on sister chromatid exchanges and micronucleus of cultured cells were studied. These results laid a foundation for all-sidedly evaluating roles of poly (ADP-ribose) polymerase inhibitors within cells.

关 键 词：苯甲酰胺 细胞 SCE PARP 酶活性 抑制剂 

分 类 号：Q26[生物学—细胞生物学]