持续静压力对人牙周膜细胞OPG及ODFmRNA表达的影响  

Effect of continuously compressive pressure on the expression of OPG and ODFmRNA in human periodontal ligament cells in vitro

作　　者：黄生高[1] 张建兴[1] 熊培颖[1] 王明朗[1] 钟孝欢[1] 

机构地区：[1]中南大学湘雅二医院,湖南长沙410011

出　　处：《临床口腔医学杂志》2006年第6期347-350,共4页Journal of Clinical Stomatology

基　　金：湖南省自然科学基金资助(03JJY4033)

摘　　要：目的:研究持续静压力(continuously compressive pressure,CCP)作用下人牙周膜细胞(human periodon-tal ligament cells,HPDLCs)护骨素(osteoprogerin,OPG)和破骨细胞分化因子(osteoclast differentiation factor,ODF)的表达,探讨其在正畸骨改建中的作用机制。方法:组织块酶消化法原代培养HPDLCs,建立压力模型,分别对细胞施以1 g/cm22、g/cm23、g/cm2顶-底轴向压力0.5 h1、.5 h、6 h1、2 h2、4 h和48 h,利用逆转录聚合酶链反应(re-verse transcription-polymerase chain reaction,RT-PCR)法检测OPG及ODF信使RNA(mRNA)的表达。结果:随着CCP作用时间的增加,HPDLCs ODF mRNA表达增强(P<0.01);OPG mRNA在加力后期明显升高(P<0.05)。随力值增加,ODF及OPG mRNA表达均增强,力值为2 g/cm2时,改变最明显(P<0.05)。结论:CCP可上调HPDLCsOPG及ODF mRNA表达。而OPG mRNA表达升高则明显滞后。Objective：To study the effect of continuously compressive pressure（CCP） on the expression of OPG and ODF in human periodontal ligament cells （HPDLCs） ,to investigate the role of osteoprogerin, （OPG） and osteoclast differentiation factor （ODF） in alveolar bone rebuilding during orthodontic tooth movement . Method： The primary HPDLCs were isolated from human periodontal ligament by explanting with enzymatic digestion with trypsin and collagenase . A pressure model was established, and 1 g/cm^2 ,2 g/cm^2 ,3 g/cm^2 top-bottom axial pressure were laid on HPDLCs for 0.5 h,1.5 h,6 h,12 h,24 h and 48 h respectively . The expression of ODF was indicated by the reverse transcription-polymerase chain reaction （RT-PCR） at messenger RNA（mRNA） level. Result：The expression of ODF mRNA was significantly increased in a time-dependent manner（P 〈 0.01 ） And the expression of OPG mRNA was also strengthed at the end of the increased pressure . In 2 g/cm^2 group the greatest expression of ODF and OPG mRNA could be detected （P 〈 0.05）. Conclusion： CCP can up-regulate the expression of OPG and ODF mRNA in Human periodontal ligament cells. But the increase of the expression of OPG mRNA was later than ODF mRNA.

关 键 词：压力 牙周膜细胞 护骨素 破骨细胞分化因子 逆转录聚合酶链反应 

分 类 号：R780.1[医药卫生—口腔医学]