用考马斯亮蓝测定植物粗提液中可溶性蛋白质含量方法的研究  

Method research of measuring soluble protein contents of plant rough extraction using Coomassie Brilliant Blue

作　　者：曲春香[1] 沈颂东[1] 王雪峰[1] 崔永华[1] 宋卫平[1] 

机构地区：[1]苏州大学生命科学学院,江苏苏州215006

出　　处：《苏州大学学报（自然科学版）》2006年第2期82-85,共4页Journal of Soochow University(Natural Science Edition)

基　　金：苏州市科委资助(SNZ-9915)

摘　　要：用木立芦荟的叶片为材料,以牛血清白蛋白为参照,对蛋白质与考马斯亮蓝G-250结合后的光吸收稳定性,不同的蛋白质溶剂、植物粗提液等对光吸收的影响进行了研究。结果表明：蛋白质与考马斯亮蓝结合后,在595nm处的光吸收值不很稳定,通过降低仪器的灵敏度可以提高光吸收的稳定性；以0.02～0.5mol·L^(-1)pH为6.8的磷酸缓冲液、0.1～1.0mol·L^(-1)pH为6.8的Tris-HCI缓冲液为蛋白质溶剂,对蛋白质与考马斯亮蓝结合后的线性无影响；芦荟叶片粗提液中的其他成分对其中可溶性蛋白质与考马斯亮蓝结合后的线性亦无显著影响。The steady of light absorption of Coomassie Brilliant Blue G-250 combined with proteins extracted from the leaves of Aloe arborescens Mill and BSA was studied. Also the influences of light absorptions of various reagents and plant rough extraction were studied in this paper. The results showed that the light absorption value was not very steady in the wave length of 595nm, after the Coomassie Brilliant Blue combined with proteins, and the stability can be promoted by lowing the sensitive of the spectrophotometer. The linearity kept very good when the reagents were changed with 0. 1mol· L^-1, pH 6.8 PBS or 0. 125 mol.L^-1, pH 6.8 tris-HCl buffer. The other materials in the plant rough extraction had not obviously influences to the Coomassie Brilliant Blue combined with proteins.

关 键 词：考马斯亮蓝 可溶性蛋白质 含量测定 方法分析 

分 类 号：Q946.1[生物学—植物学]