益生枯草芽孢杆菌MA139增殖培养基的优化  

Medium optimization for spore production of Bacillus subtilis MA139 as a probiotic

作　　者：郭小华[1] 陆文清[1] 邓萍[1] 黄德仕[1] 

机构地区：[1]中国农业大学农业部饲料工业中心,北京100094

出　　处：《中国农业大学学报》2006年第3期41-46,共6页Journal of China Agricultural University

摘　　要：为得到益生芽孢杆菌Bacillus subtilisMA139产芽孢的最佳的培养基,以摇瓶发酵的方法,用Plackett-Burman设计从11种原料中筛选出4种对芽孢产量有显著影响的因素,即玉米粉、大豆粉、蛋白胨和MnSO4.H2O,然后针对这4个主要因素,用最陡爬坡试验及中心组合设计优化产芽孢的最佳培养基。结果表明,当培养基的配方为:玉米粉3.17 g/L、大豆粉5.80 g/L、蛋白胨3.62 g/L、MnSO4.H2O 1.06 g/L、葡萄糖5 g/L、尿素3 g/L、MgSO4.7H2O 1.5 g/L和KH2PO43 g/L时,MA139发酵36 h细菌总数可以从8.32×108cfu/mL提高到3.10×109cfu/mL,芽孢率达到96%。试验表明通过统计优化培养条件可以有效提高B.subtilisMA139产芽孢的得率。Using shaking-flask fermentation in the laboratory, the ingredients influencing spore production of Bacillus subtilis MA139 were analyzed through the design of Plackett-Burman in Software JMP 5.0. The concentration of ingredients was optimized through path steepest ascent and sequent central composite design （CCD）. The ingredients concentration was： corn meal 3.17 g/L, soybean meal 5.80 g/L, peptone 3.62 g/L, glucose .5 g/L, urea 3 g/L, MgSO4- 7H2O 1.5 g/L and KH2PO4 3 g/L, The total bacterial counts were improved from 8.32 × 10^8 cfu/mL to 3.10 × 10^9 cfu/ mL after medium optimization, and the spore rate reached 96%. This study suggests that the spore concentration can be improved by the experimental designs.

关 键 词：枯草芽孢杆菌 发酵 培养基优化 试验设计 

分 类 号：Q93-335[生物学—微生物学] Q939.124