用非放射性原位杂交技术检测胃癌组织c-erbB-2和c-myc癌基因扩增的研究  

c-erbB-2 and c-myc Expression is Related with Pathological Progression of Gastric Cancer:Results of a Non-radioactive in Situ Hybridization

作　　者：王克强[1] 李雁[2] 晁储璋[1] 韩国新[1] 刘振忠[1] 东野圣伊[1] 

机构地区：[1]泰山医学院附属医院,山东泰安271000 [2]武汉大学肿瘤研究所武汉大学中南医院肿瘤科,湖北武汉430071

出　　处：《现代检验医学杂志》2006年第3期25-27,共3页Journal of Modern Laboratory Medicine

摘　　要：目的研究c-erbB-2和c-m yc癌基因在胃癌组织中扩增的意义。方法应用非放射性原位杂交技术检测81例胃癌标本中c-erbB-2和c-m yc的扩增情况。结果c-erbB-2和c-m yc在胃癌中的扩增率分别为50.6%和67.9%。c-erbB-2基因与胃癌组织分化程度、浸润深度及淋巴结转移有关(P<0.05),分化程度越差、浸润深度越深、淋巴结有转移,c-erbB-2基因表达率越高,而c-m yc癌基因与胃癌组织分化程度、浸润深度及淋巴结转移无关。两基因的扩增具有显著的相关性(χ2=7.26,P<0.01)。结论c-erbB-2和c-m yc扩增是胃癌发生过程中的一个重要的生物学因素,且两者具有协同作用。Objective To study the significance of c-erbB-2 and c-myc oncogene expression in gastric cancer. Methods 81 gastric cancer specimens were studied for c-erbB-2 and c-myc oncogene amplification using non-radloactlve in situ hybridization method. Results The amplification rates for c-erbB-2 and c-myc were 50. 6% and 67. 9%, respectively, There were significant correlation in the amplification of these two genes （x^2=7. 26, P〈0. 01）. Conclusions The amplification of c-erbB-2 and c-myc may play an important role in gastric cancer development,and these two genes may have synergistic effect.

关 键 词：胃癌 C-ERBB-2基因 C-MYC基因 原位杂交 

分 类 号：R730.231[医药卫生—肿瘤]