汉坦病毒Q32株L和M片段全基因序列分析  

Full length nucleotide sequence analysis of L and M segments of Hantavirus Q32 strain gene

作　　者：代晓霞[1] 张全福[2] 王世文[2] 楚雍列[1] 郝永华[2] 王芹[2] 修梅红[2] 张颖[2] 李德新[2] 

机构地区：[1]西安交通大学医学院免疫与微生物学系环境与疾病相关基因教育部重点实验室,西安710061 [2]中国疾病预防控制中心病毒病预防控制所传染病预防国家重点实验室

出　　处：《中华实验和临床病毒学杂志》2006年第2期56-59,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的测定汉坦病毒Q32株L、M片段的全长序列,了解其分子基础。方法设计特异的PCR引物,用RT-PCR技术分段扩增Q32株全长L、M片段,PCR产物纯化后直接测序,或用T-A克隆方法进行PCR产物克隆,然后进行遗传进化分析。结果Q32株L片段的全基因序列共6533个核苷酸,GC含量为37·38%,AT含量为62·62%,包含一个单一的开放读码框架(ORF),该读码框架从38到6493,由6456个核苷酸组成,编码2151个氨基酸,可以合成相对分子质量为2·46×105的蛋白。M片段全基因序列共3616个核苷酸,GC含量为39·44%,AT含量为60·56%,包含一个单一的开放读码框架(ORF),其读码框架从41到3448,由3408个核苷酸组成,编码1135个氨基酸,可以合成相对分子质量为1·26×105的蛋白。Q32编码的RNA聚合酶也有6个比较保守的区域。结论汉坦病毒Q32株M和L片段具有和其他汉坦病毒糖蛋白和RNA聚合酶相似的核苷酸一级结构,核苷酸序列虽有差异,但在氨基酸水平上的同源性仍很高。Objective To study the full length L and M sequence of Hantavirus Q32 strain gene and explore its molecular characters. Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced. Results The L genome segment of Q32 virus was found to be 6533 nucleotides in length. One large open reading frame was found located at bases 38 to 6493. This was predicted to encode an L protein 2151 amino acids in length with a molecular mass of 2.46 ×10^5. The M genome segment was 3616 nucleotides in length. One open reading frame was located at bases 41 to 3448. This was predicted to encode an M protein 1135 amino acids with a molecular mass of 1.26×10^5.Conclusion The nucleotides sequence of M and L segments of strain Q32 was similar to that of other Hantavirus M and L segments. Deduced amino acid sequences of glycoprotein and RNA polymerase revealed high homologue to other Hantavirus.

关 键 词：汉坦病毒 M基因片段 L基因片段 核苷酸序列分析 

分 类 号：R373[医药卫生—病原生物学]