Hela细胞导入TRIM45基因后的比较蛋白质组学研究  

Comparative Proteomics Analysis of Hela Cell Transfected with the TRIM45 Gene

作　　者：路俊仙[1] 柳亦松[1] 王跃群[1] 张才波[1] 陈平[1] 吴秀山[1] 梁宋平[1] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与发育生物学教育部重点实验室,中国湖南长沙410081

出　　处：《生命科学研究》2006年第2期103-108,共6页Life Science Research

基　　金：国家自然科学基金资助项目(30170479;30270722;30270644)

摘　　要：为了确定TRIM45基因的功能和作用机理,将其转入Hela细胞,分析细胞内蛋白质表达水平的变化.研究采用双向凝胶电泳的方法分别对转入pCMV-Tag2B空载体和重组质粒pCMV-Tag2B-TRIM45的Hela细胞全蛋白进行分离,利用PDQUEST7.2软件分析,鉴定得到15个差异点,包括8个上调点和7个下调点.为进一步研究TRIM45基因的功能奠定了良好的基础.In search of mechanisms and function of TR1M45 gene, it was introduced into Hela cells. Differential display proteomics techniques was employed to study protein changes of Hela ceils transfected with pCMV-Tag2B-TRIM45 compared with cells transfected with pCMV-Tag2B. Image analysis was carried out using PDQuest 7.2 image analysis software. 15 proteins were identified, of which 8 were up-regulated while 7 were down-regulated. These in vitro studies may be helpful in studying mechanisms that may lead to the function of TRIM45.

关 键 词：HELA细胞系 蛋白质组学 双向凝胶电泳 质谱 

分 类 号：Q297[生物学—细胞生物学] Q51