红车轴草提取物对胃癌BGC-823细胞凋亡的影响  

Effect of Trifoliumpratense L. Extract on Apoptosis of BGC-823 Cells

作　　者：郑杰[1] 刘艳平[1] 宗永立[1] 李忠魁[1] 苏文[1] 曾建国 

机构地区：[1]中南大学生物科学与技术学院细胞生物学系,中国湖南长沙410013 [2]九汇现代中药有限责任公司,中国湖南长沙410016

出　　处：《生命科学研究》2006年第2期178-182,共5页Life Science Research

摘　　要：探讨红车轴草(TrifoliumpratenseL)提取物对胃癌细胞株BGC-823的抑制增殖效应及诱导凋亡作用.我们采用不同浓度(50、100、250、500、1000mg/L)红车轴草提取物处理BGC-823细胞,采用四甲基偶氮唑盐(MTT)法检测药物对细胞的抑制作用,倒置显微镜观察细胞的形态学改变;AO/EB染色,荧光显微镜观察细胞凋亡形态;采用DNALadder观察DNA的降解;应用流式细胞仪观察细胞凋亡过程中的细胞周期的变化和细胞凋亡.结果显示在红车轴草提取物的作用下,BGC-823细胞呈凋亡改变,DNA琼脂糖凝胶电泳呈典型的凋亡特征.细胞凋亡的同时,细胞周期阻滞于G2/M期.实验结果表明红车轴草提取物能抑制胃癌BGC-823细胞增殖,能诱导胃癌细胞凋亡.To investigate whether Trifoliumpratense L. extract induces apoptosis in gastric cancer cell line BGC-823. Cell proliferation was assessed by MTI＇ assay. Morphological changes of apoptosis were observed with invert microscope apoptotic morphology was observed by staining the BGC-823 cell with AO/EB; DNA Ladder, cell cycle and apoptosis were examined by DNA agarose gel eletronphores and PI fluorescence flow cytometry （FCM）, respectively. Result showed under the influence of Trifoliumpratense L. extract, BGC-823 cell exhibited changes of apoptosis both in morphology and in the characteristics of electrophoresis. Meanwhile the cell cycle also blocked in G2/M. The study indicated Trifoliumpratense L. extract could inhibit proliferation and induce apoptosis in gastric cancer BGC-823 cell.

关 键 词：红车轴草提取物 胃癌细胞BGC-823 凋亡 增殖 

分 类 号：R739.1[医药卫生—肿瘤]