mdrl的表达在非小细胞肺癌多耐药机制中的意义  

The effection of mdrl in mechanism of drug resistance of non-small cell lung cancer

作　　者：宋晓荣[1] 李光华[1] 姬明丽[1] 

机构地区：[1]新乡医学院病理生理学教研室,河南新乡453003

出　　处：《中原医刊》2006年第12期1-2,共2页Central Plains Medical Journal

摘　　要：目的探讨非小细胞肺癌的化疗耐药性和mdrl基因表达的关系,明确mdrl在非小细胞肺癌耐药中的作用。方法应用肿瘤细胞体外培养和MTT法对91例未经化疗的非小细胞肺癌进行体外化疗药物(环磷酰胺、阿霉素、顺铂+氟尿嘧啶)敏感性测试,并用免疫组化方法进行mdrl表达的检测,并进行相关分析。结果化疗敏感性:53例(58.2%)对环磷酰胺耐药,51例(56.0%)对阿霉素耐药,42例(46.2%)对顺铂+氟尿嘧啶耐药。mdrl在91例中总的阳性表达率为58.2%。鳞癌和腺癌相比mdrl的表达差异无统计学意义(P>0.05),而且mdrl与耐药性均呈显著正相关(P<0.05),并且不同表达程度间差异也有统计学意义(P<0.05)。结论mdrl参与介导了非小细胞肺癌固有化疗耐药的机制。Objective To investigate the multidrug resistance （MDR） of non -small cell lung cancer （NSCLC） and the expression of mdrl gene. Methods 91 cases of NSCLC were tested with MTT assay to evaluate their chemoresistance. The expression of mdrl gene was examined with immunohistochemical assay. Results The percentage of drug resistance of cyclophamide, adriamycin and cisplatin ＋ fluorouracil were 58.2% ,56.0% and 46.2%. The expression percentage of mdrl gene was 58.2%. There is not significant difference in the expression of mdrl between human adeoncarcinoma and sequamous cell carcinoma （P〉0.05 ）. The correlation between the positive expression of mdrl gene and the degrees of chemoresistance to the three kinds of agents was significantly （P〈0.05）. Conclusion There is something between MDR and mdrl gene in NSCLC.

关 键 词：非小细胞肺癌 多药耐药 MDRL基因 体外药敏试验 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤] R730.53[医药卫生—临床医学]