诺帝抑制胃癌细胞系SGC-7901血管生成因子表达的实验观察  

Inhibitory Effect of Nordy on Expression of VEGF, Ephrin B2 and Eph B4 in Gastric Adenocarcinoma Cell Line

作　　者：宋文先[1] 卞修武[1] 蒋雪峰[1] 王清良[1] 

机构地区：[1]第三军医大学西南医院病理学研究所,重庆400038

出　　处：《临床消化病杂志》2006年第3期145-147,共3页Chinese Journal of Clinical Gastroenterology

摘　　要：目的探讨诺帝(Nordy)对胃癌细胞血管生成因子VEGF、EphrinB2及其受体EphB4表达的影响及其意义。方法采用细胞培养、透射电镜、免疫细胞化学和图像分析等方法观测诺帝作用前后人胃腺癌细胞系SGC-7901细胞形态及VEGF、EphrinB2和EphB4蛋白表达的变化。结果100μmol/L诺帝处理48h后,胃癌细胞核异型性降低,细胞器减少;VEGF、EphrinB2和EphB4蛋白表达水平降低。结论诺帝可抑制胃癌细胞VEGF、EphrinB2和EphB4蛋白表达,此作用可能是诺帝抗血管生成作用的重要分子基础。Objective To explore the effect of nordy on the expression of vascular endothelial growth factor（ VEGF）, Ephrin 132 and Eph I34 and its signification in gastric adenocarcinoma cell line. Methods The expression of VEGF, Ephrin 132 and Eph I34 in human gastric adenocarcinoma cell line SGC-7901 was observed 48 h after nordy treatment with immunocytochemistry and image analysis. Results The expression of VEGF, Ephrin 132 and Eph I34 was declined at protein level in SGC-7901 after treated with 100 μmol/L for 48 h. Conclusion The results suggest that nordy could inhibit the expression of VEGF, Ephrin 132 and Eph I34 in human gastric adenocarcinoma cells, which might be the important molecular mechanism of anti-angiogenesis of nordy.

关 键 词：诺帝 胃癌 血管内皮生长因子 血管生成因子 

分 类 号：R735.2[医药卫生—肿瘤]