胃癌组织中CD44v6蛋白表达和血清sCD44v6水平变化的研究  

A study on the change of the serum levels of soluble sCD44v6 and the expression of CD44v6 in the tissues of gastric tumor

作　　者：李荣华[1] 张辰 王邦茂[3] 张惠彬[4] 崔如众 王天雄[4] 李志刚[4] 

机构地区：[1]河北医科大学第三医院肿瘤科,石家庄市050051 [2]北京市上地医院内科 [3]天津医科大学总医院消化科 [4]天津汉沽医院内科

出　　处：《河北医药》2006年第5期355-358,共4页Hebei Medical Journal

摘　　要：目的研究组织中CD44v6的表达以及血清sCD44v6水平变化与胃癌生物学行为和Lauren分型的关系,为进一步探讨两者与胃癌浸润转移关系奠定基础。方法采用免疫组化SP法观察CD44v6在42例胃癌组织中的表达情况,以及应用ELISA法检测53例胃癌患者血清sCD44v6水平变化。结果CD44v6在30例正常胃粘膜组织中均不表达,在胃癌组织中表达61.9%(2642),血清sCD44v6在胃癌组(n=53)水平为(565.69±165.89)ng ml明显高于正常对照组(n=42)及胃溃疡组(n=29),无论CD44v6的表达还是sCD44v6水平均随胃癌浸润扩展、TNM分期上升及转移的发生而增高。弥漫型胃癌CD44v6表达80%(1620)明显高于肠型胃癌者45.5%(1022),并且随着浸润进展及转移程度而增加(P<0.05),同时弥漫型胃癌患者血清sCD44v6水平[(629.61±118.10)ng ml,n=22]明显高于肠型胃癌者[(520.33±101.17)ng ml,n=31],并随着远处转移的发生而增高(P<0.05)。结论组织中CD44v6表达和血清sCD44v6水平变化在胃癌,尤其是弥漫型胃癌的浸润转移的预测与监测具有临床检测意义。Objective To investigate the correlation between biological behavior in gastric tumor and the serum levels of sohlble CD44v6 and the expression of CDd4v6 in the tissues of gastric tumor in order to investigate further the possible mechanism of metastasis in gastric tumor. Methods The expression of CD44v6 in 42 cases of gastric tumor tissues was detected by immunohistochemical SP method. The serum levels of sCD44v6 in 53 patients with gastric tumor were. measured by ELISA. Results The positive rate of CD44v6 in control group and in gastric tumor tissue was 0% and 61.9% respectively.The serum sCD44v6 in gastric tumor group （ n = 53） was （565.69±165.89） ng/ ml, which was obviously higher than that in control group （ n = 42） and in gastric ulcer group （ n = 29）. The expression of CD44v6 and the levels of sCD44v6 were increased with the TNM stage, invasive depth and metastasis status （ P 〈0.05）.The serum levels of sCD44v6 in patients with gastric tumor （ n = 53） were （565.69±165.89） ng/ ml, which were significant higher than those, in control group （ n = 42） and in patients with gastric ulcer （ n = 29, P 〈 0.01 ）. The levels of serum sCD44v6 levels increased with TNM stage and metastatic status （ P 〈 0.05）. The levels of serum sCD44v6 levels in patients with diffuse-type gastric carcinoma were （629.61 ± 118.10） ng/ml, wihch were significant higher than those （520.33 ± 101.17） ng/ml in patients with intestinal-type gastric carcinoma （ P 〈 0.05） .Conclusion The expression of CD44v6 and serum levels of sCD44v6 may be of significant clinical value in predicting and monitoring the invasion and metastasis of gastric carcinoma, especially in diffuse-type gastric carcinoma.

关 键 词：弥漫型胃癌 肠型胃癌 CD44V6 SCD44V6 浸润转移 

分 类 号：R735.2[医药卫生—肿瘤]