老年雄性大鼠睾丸类固醇合成酶与间质细胞形态的变化  

The changes of steroidogenic enzymes and Leydig cell morphology in aged rat testis

作　　者：邵迎红[1] 母义明[2] 李明[2] 李江源[2] 

机构地区：[1]中国人民解放军总医院老年内分泌科,北京市100853 [2]中国人民解放军总医院内分泌科,北京市100853

出　　处：《中华老年多器官疾病杂志》2006年第1期44-47,共4页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：国家重点基础研究发展规划(G2000057008)

摘　　要：目的通过测定老年雄性SD大鼠睾丸类固醇合成急性调节蛋白(StAR)、17β-羟类固醇脱氢酶(17β- HSD)表达,观察睾丸间质和Leydig细胞结构的变化,探讨老年大鼠性腺功能减退的机制。方法用人绒毛膜促性腺激素(hCG)连续刺激3月龄和24月龄雄性SD大鼠,测定基础状态和hCG刺激后血清睾酮(T)、睾丸StAR、17β- HSDⅢ mRNA的表达,对不同年龄大鼠睾丸间质和Leydig细胞进行光镜和电镜检查。结果 (1)老年SD大鼠基础状态和hCG刺激后血清T水平和17β-HSDⅢ mRNA的表达显著低于青年大鼠,StAR mRNA表达无明显变化;(2)老年大鼠睾丸间质中成纤维细胞增生明显而Leydig细胞数量减少,并出现退行性改变,hCG刺激8 d后,青年和老年大鼠两组睾丸形态学均无明显变化。结论雄性大鼠睾丸17β-HSDⅢ mRNA表达以及Leydig细胞形态学随着增龄的明显异常可能是老年SD大鼠T合成减少的主要影响因素。Objective To investigate the possible mechanisms of decreasing steroidogenie function in the aged rat testes through determining the effects of aging on the steady-state levels of the mRNAs of testicular steroidogenic acute regulatory protein （STAR） and 17β-hydroxysteroid dehydrogenase（17β-HSD）, and observing the morphology of the testes in the aged male rats. Methods The young male rats and old male rats were treated with human chorionic gonadotropin（hCG） coritinuonsly for three times andthen the rat serum testosterone （T） levels and the mRNA levels of STAR, 17β-HSDm were determined respectively by ehemiluminescent enzyme immunosssay（CLlA） and RT-PCR. At the same time, the morphology of testis interstitial tissue and Leydig cells were studied under light microscope and electronmicroscope. Results （1）The levels of serum T and 17β-HSDm mRNA of aged SD rats decreased significantly before and after hCG stimulation, and the StAR mRNA level in aged testes was similar to that in young testis. （2） The appearance of testis in aged SD rat was gloomy and loose in texture. In contrast to the testis of young rats, the number of fibroblasts in the testis inteistitial tissue of the aged rat was significantly increased and the number and the size of Leydig cells were significantly decreased. Leydig cells in aged rats exhibited retrograde changes, The treatment of hCG had on effect on the morphological appearance of Leydig cells in both aged and young rats. Conclusion The morphological abnormalities of Leydig cells from aged testis and the reduction in the level of 17β-HSD Ⅲ are contributive to the age-related hypogonadism.

关 键 词：衰老 大鼠 睾丸 睾酮 类固醇合成急性调节蛋白 17β羟类固醇脱氢酶 形态学 

分 类 号：R730.21[医药卫生—肿瘤] R733.71[医药卫生—临床医学]