壳聚糖的抑菌活性分析及其抑菌机理的研究  

Preliminary studies on antibacterial mechanism and analysis of antibacterial activity of chitosans

作　　者：荆迎军[1] 郝友进[1] 渠晖[1] 单颖[1] 李德森[1] 杜荣骞[1] 

机构地区：[1]南开大学生命科学学院遗传工程研究室,天津300071

出　　处：《中国抗生素杂志》2006年第6期361-365,375,共6页Chinese Journal of Antibiotics

基　　金：天津市自然科学基金(No.033604911)。

摘　　要：制备10个分子量的壳聚糖,对6种细菌作抑菌实验。抑菌实验结果表明分子量在14．9～297．6ku的壳聚糖有很强的抑菌活性,此活性有随pH的降低而增加的趋势,pH5．5时最低抑菌浓度为0．03%～0．06%,Ca^(2+)、Mg^(2+)可显著降低壳聚糖对革兰阴性菌的抑菌作用。壳聚糖的抑菌机理有可能影响细胞膜的通透性。对实验结果的方差分析表明,壳聚糖的不同分子量、pH值和金属离子等外界因素都是壳聚糖抑菌活性的极显著影响因素,但菌株本身才是壳聚糖抑菌活性大小的最关键因素。Ten chitosans with different molecular weights were prepared and their antibacterial activities were examined against six bacteria. Results showed that chitosans （14.9 - 297.6ku） have stronger antibacterial activities. Chitosans showed higher antibacterial activities at low pH value. The minimum inhibitory concentration of chitosans ranged from 0.03% - 0.06%. The antibacterial mechanism of chitosans might be related to the penetration mechanism of the cell membrane. Different molecular weights, pH value and ion can strongly affect the antibacterial activities of chitosans, but the type of bacteria was the key effecting factor.

关 键 词：壳聚糖 抑菌 机理 

分 类 号：Q636.1[生物学—生物物理学]