加减温胆汤的抗抑郁功效  

Antidepressant function of modified wendan decoction

作　　者：武丽[1] 张丽萍[1] 叶庆莲[1] 何清平[1] 

机构地区：[1]广西中医学院中医基础理论教研室,广西壮族自治区南宁市530001

出　　处：《中国临床康复》2006年第23期63-64,69,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察以调理脾胃法组成的中药复方加减温胆汤(解郁1号)对抑郁大鼠海马、皮质5-羟色胺、去甲肾上腺素含量的影响。方法:实验于2004-03/2004-04在广西中医学院中心实验室完成。SD大鼠40只,按随机表随机分为正常组、模型组、阿米替林组、加减温胆汤12,6g/(kg·d)剂量组,各8只。加减温胆汤(解郁1号)为半夏,竹茹,枳实,茯苓,柴胡,石菖蒲,合欢花,各10g,等组成,中药购于广西中医学院附属一院中药房,经中药植化室鉴定后,水煎浓缩为1.5g/mL,置4℃冰箱内备用。采用慢性不可预见性应激诱导抑郁大鼠模型,各组在造模同时即开始给药,分别胃饲阿米替林10mg/(kg·d)(蒸馏水配置,浓度为1g/L),加减温胆汤提取液12,6g/(kg·d),模型组、正常组胃饲生理盐水(6mL/kg),每日8∶00开始,除正常组外,各组接受21d长期慢性刺激。21d后进行大鼠旷场行为的测定,比较各组大鼠水平运动次数和垂直运动次数的变化;采用高效液相电化学法测定海马、皮质区5-羟色胺、去甲肾上腺素含量。观察中药复方加减温胆汤(解郁1号)的抗抑郁效应。结果:于实验第2周,正常组、阿米替林组各死亡1只大鼠,解剖无特殊发现,最终进入结果分析38只。①模型组与正常组比较,水平运动次数和垂直运动次数均明显减少(P<0.01,0.05)。加减温胆汤12,6g/(kg·d)剂量组旷场活动明显增强,与模型组相比,差异有显著性意义(P<0.05)。②模型组大鼠脑组织的5-羟色胺、去甲肾上腺素含量均低于正常组(P<0.01,0.05);与模型组比较,加减温胆汤12,6g/(kg·d)剂量组、阿米替林组5-羟色胺、去甲肾上腺素含量多明显升高(P<0.05,0.01);加减温胆汤12,6g/(kg·d)剂量组、阿米替林组之间5-羟色胺含量比较未见显著性变化(P>0.05)。结论:加减温胆汤(解郁1号)上调脑内海马、皮质区5-羟色胺、去甲肾上腺素水平,可能是其抗抑郁作用的基础。AIM： To investigate the effect of modified wendan decoction （WD）, a complex prescription with the function of adjusting spleen and stomach, on content of 5-hydroxytryptamine （5-HT）, norepinephrine （NE） in cortex and hippocampus of rats with depression. METHODS： This experiment was conducted in the central laboratory of Guangxi College of Traditional Chinese Medicine from March to April 2004. Forty SD rats were selected and randomly divided into normal group, model group, amitriptyline group, high and low-dose WD group with 8 rats in each group according to the normal random table. WD （provided by the Department of Chinese herbs, First Hospital affiliated to Guangxi College of Traditional Chinese Medicine） was made of banxia, zuru, zhishi, fuling, caihu, shichangpu and hehuanhua with 10 g, respectively and concentrated to 1.5 g/mL after decocted by water to preserve in the refrigerator at 4℃, Chronic mild unpredictable stress was applied to establish the rat models of depression; meanwhile, every group was given amitriptyline 10 mg/kg per day （with a concentration of 1 g/L, prepared by distilled water） and WD extraction 12, 6 g/kg; the normal, and model groups were given normal saline （6 mL/kg） at 8：00 in the morning. Except the normal group, every group was given the chronic mild unpredictable stress for 21 days. Twentyone days later, open-field test was used to estimate the depressed behavior, and compared times of horizontal motion and vertical motion in each group; the content of 5-HT and NE in cortex and hippocampus was measured with HPLC electrochemistry; the antidepressant effect of WD was observed. RESULTS： A total of 38 rats were involved in the result analysis except for 2 rats of normal and amitriptyline group died without any special found at the 2^nd week. ①The scores in horizontal motion and vertical motion of the model group were all significantly lower than the normal group （P 〈 0.05, P 〈 0.01）. Compared with the model group, the open-field test of th

关 键 词：精神治疗药物 抑郁症/中药疗法 中药药理学 复方合剂 汤剂 

分 类 号：R971.4[医药卫生—药品] R289.5[医药卫生—药学]