电针对新生鼠缺氧缺血脑组织神经细胞凋亡及神经生长因子蛋白表达的影响  

Effect of electroacupuncture on neuronal apoptosis and protein expression of nerve growth factor in brain tissues of newborn rats with hypoxic-ischemic injury

作　　者：刘振寰[1] 潘佩光[1] 祁岩超 赵勇 柴铁劬[3] 唐纯志[3] 王琴玉[3] 杨君军[3] 林锦泉[3] 

机构地区：[1]广州中医药大学附属南海妇产儿童医院小儿神经康复科,广东省佛山市528200 [2]广州市肿瘤医院内科,广东省广州市510095 [3]广州中医药大学针灸推拿学院实验室,广东省广州市510405

出　　处：《中国临床康复》2006年第23期114-118,i0002,共6页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察电针治疗对新生鼠缺氧缺血脑神经细胞凋亡及神经生长因子蛋白表达,肢体功能的影响,分析该种影响是否与针刺时间有关。方法:实验于2005-08/10在广州中医药大学实验动物中心清洁级实验室及针灸推拿学院实验室完成。①选用7d龄新生SD幼鼠109只。将大鼠结扎左侧颈总动脉后恢复2h,置于透明密闭容器中,并入37℃恒温水中以1L/min的速度通入低氧气体(体积分数0.08氧气和0.92氮气),2.5h后将动物取出,将存活者继续保温1h时后作行为测定,翻身不能、平衡异常或左旋者视为成功的模型(72只)。②将其余24只大鼠设为假手术组:只分离左颈总动脉后不结扎,亦不作低氧处理。将造模成功的大鼠72只按随机抽签法分为3组:缺氧缺血+针刺Ⅰ组、缺氧缺血+针刺Ⅱ组、模型组,每组24只。选用大鼠百会、患侧颞Ⅰ针、内关、曲池、足三里、涌泉。缺氧缺血+针刺Ⅰ组于造模后24h开始针刺,前7d仅针四肢部(位,第8天开始加针头部(位。缺氧缺血+针刺Ⅱ组造模后第8天开始针刺,头、体针同步。两组均以针灸针先在内关、涌泉速刺微出血,不留针,然后针头部及曲池、足三里。百会、颞Ⅰ针及曲池、足三里接G6805-Ⅱ电针仪,连续波,频率5～10Hz,持续10min。1次/d,缺氧缺血+针刺Ⅰ组连续针刺20d,缺氧缺血+针刺Ⅱ组连续针刺13d。假手术组针刺方法与缺氧缺血+针刺Ⅰ组相同;模型组只造模,不予以任何治疗。③于术后第7,14,21天,将胶布粘于大鼠两前爪腹侧面后,记录其撕去胶布所用时间。④术后第21天各组随机选取10只大鼠作以下检测:分别采用原位末端标记法及免疫组化方法测定大鼠额页皮质与海马区神经元凋亡及海马区神经生长因子表达,用图像分析仪在光镜下(×400)计数神经细胞凋亡数目及神经生长因子免疫阳性细胞表达数目。⑤率的比较采用χ2检验,组间多均数比较采用方差分AIM： To study the effect of electroacupuncture on apoptosis of hypoxic-ischemic cerebral neural cells, protein expression of nerve growth factor and the function of limbs in newborn rats, and analyze whether the effect is related with the time of acupuncture. METHODS： The experiment was conducted in the Laboratory of Acupuncture ＆ Massage as well as the Clean Laboratory of Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine from August to October 2005. ①109 young SD rats aged 7 days were selected. Rats recovered for 2 hours after ligation of left common carotid artery and then were put into a hyaline airtight container, in which there was homeothermal water of 37℃, and hypoxia gas （including 8% of O2 and 92% of N2 was infused in the container at the speed of 1 L/min. Animals were taken out at 2.5 hours later, and the survival rats received behavioral assessment following 1-hour incubation. Rats unable to roll over, abnormal in balance or kept on turning left were taken as successful models （n=72）. ②The rest 24 rats were taken as the sham-operation group： rats were only separated of the left common carotid artery without ligation or hypoxia treatment. Rats of successful modeling were randomly divided into 3 groups： hypoxic-ischemic ＋ acupuncture Ⅰ group （group A）, hypoxic-ischemic ＋ acupuncture Ⅱ group （group B） and model group with 24 rats in each group. The acupoints of Baihui, Ⅰ needle of temple in affected lateral, Neiguan, Quchi, Zusanli, Yongquan were selected. Rats in group A were acupunctured at 24 hours after modeling, and only acupoints in four extremities were acupunctured in the first 7 days, while acupoints in head were also acupunctured from the 8^th day. Rats in group B were acupunctured at both head and body on the 8^th day. Rats of both groups were acupunctured rapidly at Neiguan and Yongquan with little blood and without retention, then head-acupoints, Quchi and Zusanli were acupunctured. Baihui, Ⅰ needle of temple, Quchi

关 键 词：缺氧缺血 脑/针灸疗法 电针 细胞凋亡 神经生长因子类 

分 类 号：R745.1[医药卫生—神经病学与精神病学]