急性毒蕈肝中毒家兔模型制备的实验研究  

Experiment Research on Establishing Rabbit Model of Acutely Poisoned Liver by Poisonous Mushroom

作　　者：杨宁[1] 肖桂林[1] 宋昆[1] 

机构地区：[1]中南大学湘雅医院,长沙410008

出　　处：《实验动物与比较医学》2006年第2期88-90,共3页Laboratory Animal and Comparative Medicine

基　　金：湖南省自然科学基金资助项目(NO:05JJ30175)

摘　　要：目的通过采用灰花纹鹅膏菌毒素致家兔急性毒蕈中毒,观察中毒家兔肝脏的生化指标及病理改变,以获得急性毒蕈中毒的动物模型。方法选用家兔16只,随机分成4组(每组4只),实验组Ⅰ￣Ⅲ分别用灰花纹鹅膏菌毒素0.05、0.1、0.2g/kg灌胃。对照组,生理盐水灌胃。隔日取血样查肝功能,观察120h后处死,取肝组织作病理检查。结果对照组家兔无肝脏的生化指标及病理改变,实验组动物的肝功能均有不同程度的损害,肝脏病理变化为肝细胞不同程度的变性、坏死,汇管区或肝小叶内有炎细胞浸润。结论鹅膏菌毒素对家兔的肝功能有明显的损害作用,且损害作用随毒素剂量的增加而愈显著。制备急性毒蕈中毒的合适动物模型,宜选择给予0.1g/kg剂量的毒素。Objective To observe the changes of the biochemical index and pathology of the rabbit liver acutely poisoned by poisonous mushroom. Methods The rabbits were i.g with toxins from Amanita filiginea at the dosage of 0.05 , 0.1, 0.2 g/kg respectively, and the matched control with 0.9%NS. The biochemical index of liver were observed in every two days, the rabbits were sacrificed after 120 hours, and the pathologic changes of the liver were examined. Results Liver function of the control is normal Liver function of test groups were damaged with different degree, degeneration or necrosis happen in liver cell, inflammatory cells are in a vessel or a leaf. Conclusion Amatoxins can damage to liver function of rabbit, and the injury is more obvious depended on the amount of toxins. For making the proper animal model, acutely poisoned by Amanita fuliginea, should choose to give the toxins at the dosage of 0.1 g/kg.

关 键 词：急性毒蕈中毒 鹅膏毒素 动物模型 

分 类 号：R-332[医药卫生]