海南黄牛和徐闻黄牛线粒体DNA的多态性及其品种分化关系  

MITOCHONDRIAL DNA POLYMORPHISM IN XUWEN YELLOW CATTLE AND HAINAN YELLOW CATTLE

作　　者：聂龙[1] 陈永久[1] 王文[1] 宿兵[1] 兰宏[1] 张亚平[1] 杨关福[1] 张细权[1] 李加琪[1] 

机构地区：[1]中国科学院昆明动物研究所细胞与分子进化开放研究实验室,华南农业大学动物科学系

出　　处：《Zoological Research》1996年第3期269-274,共6页动物学研究（英文）

基　　金：国家杰出青年科学基金;云南省应用基础研究重大项目

摘　　要：本文以ＡｐａⅠ、ＡｖａⅠ、ＢａｍＨⅠ、ＢｇｌⅠ、ＢｇｌⅡ、ＤｒａⅠ、ＥｃｏＲⅠ、ＥｃｏＲⅤ、ＨｉｎｄⅢ、ＨｐａⅠ、ＰｓｔⅠ、ＳａｌⅠ、ＳｃａⅠ和ＸｈｏⅠ等１４种限制性内切酶分析来自海南岛的海南黄牛和雷州半岛的徐闻黄牛的线粒体ＤＮＡ限制性片段长度多态性（ｍｔＤＮＡＡＲＦＬＰ）。结果只有一种限制性内切酶（ＳａｌⅠ）在海南黄牛中检测到多态性，并且其中的Ｃ型（１５．０，１．３）尚未见报道。我们的结果还显示，两个品种６个个体的ｍｔＤＮＡ基因单倍型全部表现为Ａ型，即瘤牛的血统。徐闻黄牛和海南黄牛ｍｔＤＮＡ极低的遗传变异度表明两个品种的亲缘关系很近，从而在分子生物学水平为其合称为雷琼黄牛提供了佐证。Fourteen restriction endonucleases, Apa Ⅰ, Ava Ⅰ, BamH Ⅰ, BglⅠ, BglⅡ, Dra Ⅰ,EcoR Ⅰ, EcoRⅤ, HindⅢ, Hpa Ⅰ, Pst Ⅰ, Sal Ⅰ, Sca Ⅰ and Xho Ⅰ, were used to studythe mtDNA restriction fragment length polymorphism (RFLP) in 6 individuls of the Hainan and Xuwen yellow cattle from Hainan and Guangdong. Only (SalⅠ ) out of 14 restrictionenzymes disclosed mtDNA polymorphism within the Hainan yellow cattle, of which the C pattern (15.0, 1.3) has not been reported previously. The restriction patterns generated by 13 of the enzymes in Xuwen yellow cattle were identical with those in Hainan yellow cattle. The halotypes of the 6 individuals were almost A-A-A-A-A-A-A alone represented by Bosindicus. Our results showed monophenetic maternal origins of these two breeds of yellow cattle, and suggested that the genetic diversity within the two breeds is remarkably scarce. The low variation and monotoneous population genetic structure indicated a close relationship between the tWo breeds, which supported the hypothesis that Hainan yellow cattle and Xuwen cattle were from one breed named Leiqiong yellow cattle.

关 键 词：黄牛 徐闻黄牛 海南黄牛 DNA多态化 品种 分化 

分 类 号：S823.81[农业科学—畜牧学]