Tween类胶束体系中水杨酸铜络合物的SOD样活性  

SOD-like Activity of Copper Salicylate Complex in Tween Micellar Systems

作　　者：刘艳[1] 张学东[1] 鲁纯素[1] 

机构地区：[1]北京医科大学药学院

出　　处：《生物化学与生物物理学报》1996年第1期38-42,共5页

基　　金：国家自然科学基金

摘　　要：合成了水杨酸铜·乙醇络合物，提纯了Ｔｗｅｅｎ－２０、Ｔｗｅｅｎ－４０，Ｔｗｅｅｎ－６０．Ｔｗｅｅｎ－８０，并测定了它们在ｐＢ７．４的磷酸缓冲液中的ＣＭＣＯ。用Ｃｙｔ，ｃ－ＨＸ－ＸＯ法分别测定了水杨酸铜·乙醇络合物清除超氧阴离子自由基的活性，不同浓度的单纯表面活性剂对Ｏ２的影响，以及表面活性剂与水杨酸铜·乙醇络合物协同清除Ｏ２的作用。观察到在胶束体系中水杨酸铜·乙醇络合物的ＳＯＤ样活性明显高于缓冲液体系无表面活性剂时的ＳＯＤ样活性，表面活性剂本身也具有清除Ｏ２的功能。抗红细胞膜脂质过氧化实验也得到类似结果。Copper salicylate-ethanol complex was synthesized and its composition was analysed. The four surfactants(Tween-20, Tween-40, Tween-60, Tween-80) were purified. Their critical micelle concentrations (OMO) in phosphate buffer (pH=7.4) were measured. The SOD-like activity of copper salicylate complex was assayed using the cytochrome-c reduction method. The scavenging effecte of the four surfactants on O2 and OH were investigated. The cooperative effect of the complex with surfactants and micelles was studied in detail. An activity enhancement of the complex was observed in the presence of surfactants. The four surfactants themselves display SOD-like actviities to a certain extent. It was shown that the complex and the four surfactants have apparently positive cooperative actions and similar results were obtained in the experiment for anti-lipid peroxidation of red cell membrane. These results might be explained by the fact of micellar catalysis and the copper salicylate-Tween system was suggested fo be used as antiinflammatory drugs.

关 键 词：Tween类 胶束系 水杨酸 铜络合物 SOD 活性模拟 

分 类 号：R914[医药卫生—药物化学] Q554.9[医药卫生—药学]