黄芪丹参复方成分对一氧化氮合成阻滞孕鼠胎盘滋养细胞基质金属蛋白酶9 mRNA表达影响研究  

Effect of Components of Mongolian Milkvetch Root-Dan-Shen Root Compound on Expression of Matricial Metal Protease-9 (MMP-9) mRNA on Placental Nourish Cells of Nitrogen Oxide Synthesis Blocking Pregnant Rats

作　　者：王若光[1,2] 刘惠萍[2] 刘小丽[2] 曾润清[2] 秦明春[2] 尤昭玲[2] 

机构地区：[1]中南大学生物科学与技术学院分子生物学中心,湖南长沙410008 [2]湖南中医学院中西医结合系

出　　处：《实用预防医学》2006年第3期477-479,共3页Practical Preventive Medicine

基　　金：国家自然科学基金(C30000225);国家中医药管理局基础研究项目(2000-J-T-17);湖南省教委青年项目(01B014)资助。

摘　　要：目的探讨黄芪丹参复方成分影响胎盘血供的分子机理,为临床有效防治胎盘血供不足所致妊娠并发症提供思路。方法提取分离有效中药黄芪、丹参复方成分,运用NOS(一氧化氮合酶)阻滞剂L-精氨酸甲酯(L-NAME)制作一氧化氮合成阻滞大鼠模型,妊娠18d取胎盘组织,核酸原位杂交技术检测胎盘滋养细胞基质金属蛋白酶9(MMP-9)mRNA表达。结果模型组MMP-9mRNA表达明显增加,明显高于空白组和中药黄芪丹参治疗组,中药黄芪丹参治疗组与空白组比较差异无显著意义(P>0.05)。结论黄芪丹参复方成分可能通过抑制胎盘滋养细胞MMP-9mR-NA表达,阻抑蜕膜崩解、防止胎盘后出血,而有利于胎盘滋养细胞侵入及妊娠维持。Objective To provide an effective prevention and treatment of pregnant complications caused by insufficient placental blood supply, the molecular mechanism of Mongolian milkvetch root - Dan- shen root compound that influencing placental blood supply was investigated. Methods The effective components of Mongolian milkvetch root - Dan- shen root were separated and abstracted. The L- arginine methyl ester （L - NAME） nitrogen oxide synthesis blockade model was established. The placenta tissue samples were examined on the 18th day during pregnancy to inspect the expression of placental nutrient cells＇ MMP- 9 mRNA by nucleic acid in situ hybridization. Results The expression of MMP- 9 mRNA of the model group was significantly higher than that of the blank group and the Mongolian milkvetch root - Dan - shen root treatment group （P〈0.05）, however, the treatment group was similar with the blank group （P〉0.05）. Conclusions The components of Mongolian milkvetch root - Dan- shen root compound may restrain the degeneration of decidua and prevent retroplacental blooding and then keep the pregnancy going on through restraining the expression of nutrient cell MMP- 9 mRNA.

关 键 词：一氧化氮 胎盘 滋养细胞 基质金属蛋白酶类 黄芪 丹参 

分 类 号：R-332[医药卫生]