肽YY3-36对饮食诱导肥胖大鼠食欲调节的研究  

EFFECTS OF PEPTIDE YY3-36 ON APPETITE REGULATION TO DIETARY INDUCED OBESITY RESISTANT RATS

作　　者：杨年红[1] 王重建[1] 许明佳[1] 毛丽梅[1] 刘烈刚[1] 孙秀发[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生系,武汉430030

出　　处：《营养学报》2006年第3期226-229,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30371213)

摘　　要：目的:探讨高脂饲料诱导肥胖抵抗大鼠热能摄入量减少的机制。方法:雌性SD大鼠36只,按体重随机分为高脂实验组和基础对照组,分别给予高脂饲料和基础饲料13w。13w末根据体重将高脂饲料组再分为饮食诱导肥胖(dietaryinducedobesity,DIO)和饮食诱导肥胖抵抗(dietaryinducedobesityresistant,DIO-R)组,比较各组大鼠能量摄入水平,血浆PYY3-36、NPY浓度,下丘脑匀浆NPY含量及下丘脑Y2-RmRNA表达水平的差异。结果:DIO-R大鼠累积热能摄入量低于DIO大鼠(P<0.01),与对照组相比无显著性差异(P>0.05);DIO-R大鼠血浆PYY3-36浓度显著高于DIO和对照组大鼠,下丘脑匀浆NPY含量显著低于DIO大鼠(P<0.01);DIO-R大鼠下丘脑Y2-RmRNA表达水平低于DIO和对照组大鼠(P<0.01)。结论:高脂饲料喂养下,SD大鼠表现为明显的肥胖易感性差异,这种差异与PYY3-36和NPY的食欲调节密切相关,血浆PYY3-36水平的升高与下丘脑NPY水平的下降减少了能量的摄入,从而抑制了体重的过度增加。Objective： To explore the cause of decreasing energy intake of high-fat diet induced obesity resistant rats. Method- Thirty-six female SD rats were randomly divided into high-fat diet group （n=27） and control group （n=9）, and given either high-fat diet or chow for 13w. Then the high-fat diet group was subdivided into dietary induced obesity （DIO） and dietary induced obesity resistant （DIO-R） groups according to the final body weight. Weight gain, energy intake, and the plasma concentration of PYY3-36, NPY, the hypothalamic NPY levels and Y2-R mRNA were measured and compared. Results： The total energy intake was lower significantly in DIO-R rats than that in DIO rats （P〈0.01）, while no significant difference was found between DIO-R and control group （P〉0.05） . The concentration of plasma PYY3-36 was significantly higher in DIO-R rats than that in DIO and control group （P〈0.01）, while the concentration of hypothalamic NPY was lower significantly in DIO-R than that in DIO group （P〈0.01）. The expression level of Y2-R mRNA was higher significantly in DIO-R group than that in DIO and control group in hypothalamus （P〈0.01）. Conclusion： On the high-fat diet, SD rats showed different susceptibility to obesity and energy intake, DIO-R rats decreased energy intake by increasing the level of circulating PYY3-36 and reducing the level of hypothalamic NPY .

关 键 词：肥胖 肥胖抵抗 PYY3—36 NPY Y2受体 

分 类 号：R151[医药卫生—营养与食品卫生学]