多发梗死痴呆动物模型的改进与评价  

Improvement and Evaluation on Model of Multiple Infarction Dementia

作　　者：王彤[1] 于建春[2] 姜文[1] 刘存志[2] 韩景献[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第一附属医院,天津300193

出　　处：《天津中医药》2006年第3期234-236,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(30472242)。

摘　　要：[目的]对栓子注入法造成多发梗死痴呆(MID)模型加以改进并进行评价。[方法]颈外动脉逆行插管注入血栓建立MID大鼠模型,观察大鼠大脑皮质及海马的病理形态变化,同时应用Morris水迷宫检测MID大鼠的空间学习记忆的变化。[结果]1)病理形态学显示:MID大鼠局部脑区可见多发软化坏死灶,大脑皮质变薄,细胞数目减少,海马区存在广泛的细胞脱失现象,细胞排列稀疏、紊乱。2)行为学检测显示:MID大鼠学习记忆的获得、保持及再学习的能力存在严重障碍,学习记忆的过程延长。[结论]经行为学及形态学的检测,证实了栓子注入法造成多发梗死痴呆模型的可靠性和可重复性。[Objective] To improve and evaluate the model of multiple infarction dementia （MID） induced by thrombus injection. [Methods] MID was established by inverse injection of thrombus into external carotid artery to observe the pathologic changes in hippoeampus and cortex region of brain and the ability of spatial learning and memory with Morris Water Maze （MWM）. [Results] The pathologic examination showed multiple infarcted focuses in MID group in brain region. The amount of neural cell decreased in cortex,severe cell lost and cell arrangement disorder in hippoeampus region were observed. Behavior test sbowed that in MID group some severe disturbance existed in the ability of learning acquisition and memory retaining , and the course of learning and memory is delayed. [Conclusions] MID established by thrombus injection was feasible and reproducible.

关 键 词：多发梗死痴呆 皮质 海马 MORRIS水迷宫 

分 类 号：R749.16[医药卫生—神经病学与精神病学]