脂多糖信号受体CD14、TLR4在人牙髓组织和牙髓成纤维细胞中的表达  

Expression of lipopolysaccharides signal receptors CD14 and TLR4 in human dental pulp tissue and fibroblasts

作　　者：张琛[1] 侯本祥[1] 李玉晶[2] 张敏[2] 

机构地区：[1]首都医科大学附属北京口腔医院牙体牙髓科,100050 [2]首都医科大学口腔医学研究所,100050

出　　处：《中华口腔医学杂志》2006年第6期360-363,共4页Chinese Journal of Stomatology

基　　金：北京市科委科技新星计划基金资助项目(H013610290113)

摘　　要：目的研究人牙髓组织和牙髓成纤维细胞中脂多糖(LPS)信号受体CD14、TLR4的表达特点,探讨牙髓炎症组织中LPS的信号转导途径。方法采用免疫组化染色法观察健康和炎症牙髓组织中CD14、TLR4的表达情况;应用直接免疫荧光标记法,采用流式细胞术检测体外培养人牙髓成纤维细胞在LPS刺激前后的CD14、TLR4阳性细胞率和细胞表面平均荧光强度。结果正常牙髓组织中未见CD14、TLR4阳性细胞;炎症牙髓组织中可见大量CD14、TLR4阳性细胞,CD14、TLR4阳性细胞率差异无统计学意义(P>0.05)。牙髓成纤维细胞经LPS刺激后,TLR4平均荧光表达强度和TLR4阳性细胞率均显著增高(P<0.05),而CD14在LPS刺激前、后均无表达。结论炎症牙髓组织中CD14、TLR4的阳性表达,提示LPS可能通过CD14、TLR4信号受体在牙髓炎症组织中发挥作用,而牙髓成纤维细胞在LPS刺激后仅表达TLR4,表明LPS可能通过TLR4对牙髓成纤维细胞发挥作用。Objective To investigate the expression of lipopolysaccharides （LPS） receptors CD14 and TLR4 in normal and inflamed human dental pulp tissue and fibroblasts and to determine the signal transduction pathway of LPS in pulpitis. Methods CD14 and TLR4 expression in healthy and inflammatory pulps was observed by immunohistochemistry. The rates of CDI4 and TLR4 positive cells and the mean fluorescence intensity in invitro cultured pulp fibroblasts before and after being stimulated by LPS. Results CD14 and TLR4 were not detected in healthy pulp tissues, but they were strongly positively stained in inflammatory pulps. Ratio of TLR4 positive cells and mean fluorescence intensity produced by the cells stimulated by LPS increased significantly compared with normal pulp cells. However, there were no CD14 expression in human dental pulp cells before and after LPS stimulation. Conclusions This study revealed a significant increase in the expression of CD14 and TLR4 in inflamed human dental pulp tissue. Human dental pulp cells could be induced by LPS to express TLR4, which may play an important role in the process of pulpitis.

关 键 词：牙髓炎 脂多糖类 抗原 CD14 流式细胞术 

分 类 号：R781.3[医药卫生—口腔医学]