共刺激分子B7-1、B7-2在大鼠热缺血再灌注肝脏表达意义的研究  

Expression of costimulatory molecule B7-1 and B7-2 in warm ischemia/reperfusion(I/R) injury of liver in rats

作　　者：纪志鹏[1] 徐克森[1] 刘毅[1] 刘钊[1] 

机构地区：[1]山东大学齐鲁医院普外科肝胆血管外科研究所,山东济南250012

出　　处：《中国现代普通外科进展》2006年第3期151-153,共3页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然科学基金资助项目(Y2003c15)

摘　　要：目的:探讨共刺激分子B71和B72在大鼠肝脏热缺血伤再灌注损伤模型中的表达情况及其免疫学意义。方法:将雄性Wistar大鼠随机分为3组:A组(缺血30min再灌注24h)、B组(缺血60min再灌注24h)、C组(假手术组)。模型制备参考Ohmorid的方法。分别取模型之肝左叶,采用实时反转录聚合酶链反应(PTPCR)检测B71和B72在3组肝左叶组织中mRNA表达情况。结果:B71和B72mRNA在C组以极低水平表达,而在A组、B组表达却明显增多(P<0.01),且B组高于A组(P<0.05)。结论:热缺血再灌注之肝脏通过上调B71和B72的表达增加了肝脏的免疫原性。Objective：To Investigate the expression of costlmulatory molecule B7-1 and B7-2 by warm ischemia/reperfusion（1/R）injury In rat liver and the meening of ImmunolosY. Methods ： Male Wistar rats were randomly dlvicted Into 3 groups：A（ischemia 30 mins, referfusion 24h）, B（ischemia 60 mins,referfusion 24h）,C（sham operation group）. A model of hepatic warm I/R in rats was establlshed according to the method of Ohmorid. The expression of B7-1 and B7-2 mRNA was analyzed by real-time reverse transcription polyrnerase chain reaction（RT-POR）, Results ：Oompared with the sham operation group,the expression of B7-1 and B7-2 mRNA increased sisnificently In the rats unclersoing I/R procedure（P〈0.01）.Conclusion：Warm ischemia/reperfusion injury may increase the Immunosenicity of the liver by upresulating the costimulatory molecule BT-1 ancl B7-2.

关 键 词：共刺激分子 再灌注损伤 肝移植 大鼠 Wistar 

分 类 号：R735.7[医药卫生—肿瘤]