巨噬细胞刺激蛋白受体过表达对癌细胞侵袭能力的初步研究  

Preliminary study on effects of macrophage stimulating receptor overexpression on the invasive ability of human colorectal cancer

作　　者：杜卫东[1] 何超[1] 王达[1] 黄学锋[1] 毛伟芳[1] 姚文琴[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院临床研究所中心实验室 [2]3浙江大学医学院附属邵逸夫医院临床研究所中心实验室,杭州10016

出　　处：《中华消化杂志》2006年第5期304-307,共4页Chinese Journal of Digestion

基　　金：国家自然科学基金(30471986);浙江省高校科研资金计划项目(20050854)

摘　　要：目的研究巨噬细胞刺激蛋白受体(MST1R)过表达对结肠癌细胞侵袭能力的影响。方法将携有野生型 MST1R(wt-MST1R)cDNA 的质粒 pDR2-wt-MST1R 转染入结肠癌细胞株 RKO,挑选稳定转染克隆。以过河实验和趋化运动实验检测二者的移动能力,以基质浸润实验检测浸润能力,以 Western 印迹检测 E-钙粘连蛋白表达的变化。结果转染并高表达 wt-MST1R 后,RKO 细胞的趋化移动能力明显增加(P<0.01)。过河实验中转染组过河时间为(42.50±4.12)h,而未转染组与载体对照组分别为(69.50±2.52)h 与(70.50±3.42)h(P<0.01)。基质浸润实验中转染组47.90±6.82/视野,未转染组与载体对照组分别为25.90±4,56/视野与26.50±5.36/视野(P<0.01)。转染wt-MST1R 后,E-钙粘连蛋白表达降低(P<0.05)。结论 wt-MST1R 高表达可降低 E-钙粘连蛋白表达,降低肿瘤细胞间的黏附性,增加结肠癌细胞株 RKO 的侵袭能力。提示 MST1R 高表达可能是结肠癌的浸润转移机制之一。Objective To investigate the roles of overexpression of macrophage stimulating 1 receptor （MST1R） on motile and invasive ability of human colorectal cancer cell. Methods A eucaryotic expression vector pDR2 containing full-length wt-MST1R cDNA was transfected into the colorectal cancer cell line RKO, and a stable expressive clone was selected. Then the motile ability was tested by crossing-river test and transwell migration assay, the invasive ability was evaluated by matrigel invasion method, and expression of E-cadherin was detected by Western-blot, respectively. Results Motile ability of human colorectal cancer cell line RKO was significantly promoted by transfecting wt-MST1R （P 〈0.01）. There were statistical differences of crossing-river time among transfected group （42.50 h± 4.12 h）, parental group （69.50 h± 2. 52 h） and control group （70.50 h± 3.42 h）. By matrigel invasion test, the penetrated cells were 47. 90± 6.82/field in transfected group, 25.90 ± 4.56/field in parental group and 26.50±5.36/field in control group, respectively （P 〈 0.01）. The expression of E-cadherin was significantly decreased after pDR2-wt-MST1R transfection. Conclusions Overexpression of wt-MST1R decreased the expression of E-cadherin and intercellular adhension, meanwhile induced promotion of migration/invasion ability. These results indicated that abnormal accumulation of MST1R might play potential roles in invasion and metastasis of colorectal cancer.

关 键 词：巨噬细胞刺激蛋白受体 E钙粘连蛋白 结肠肿瘤 肿瘤浸润转移 

分 类 号：R735.35[医药卫生—肿瘤]