一种有效回收短片段PCR产物的方法  

A Effective Method: Extracting Short Fragment Products of PCR

作　　者：罗立廷[1] 王珏[1] 姚琴[1] 常俊丽[1] 郑重 何光源[1] 

机构地区：[1]华中科技大学中英HUST-RRes基因工程和基因组学联合实验室,湖北武汉430074 [2]湖北省洪湖市第一中学,湖北洪湖437200

出　　处：《生物技术》2006年第3期47-48,共2页Biotechnology

基　　金：国家"973"计划项目(No.2002CB111302)

摘　　要：目的:为了有效地回收小于200bp的短片段PCR产物。方法:研究了在-20℃条件下,用无水乙醇和3mol/l醋酸钠共沉淀短片段的PCR产物的方法。结果和结论:用这种共沉淀PCR产物的方法,它能够有效地回收小于200bp以下的DNA片段,并且回收到的片段能够有效的进行酶切反应和T-载体连接反应。这种方法也能够回收酶切后的短DNA片段,并对后来的连接反应没有影响。这种共沉淀回收短片段DNA方法相对于其他方法来不仅具有可行性,而且有经济和操作简单的优点。Objectives：To effectively extracted the short fragment products of PCR, less than 200bp, these products of PCR were co- precipitated with absolute ethanol and 3mol/l sodium acetate together at - 20℃, by which the extracted products of PCR could be digested by restriction endoenzyme and ligated to the T- vector by the T4. The short segment DNA, digested by restriction endoenzyme, could be ligated to other short segment DNA and it has not any bad influences on the ligated effect subsequently. The method of co - precipitated for extracted short segment DNA was feasibility and had other advantages, such as economical and convenient.

关 键 词：短片段DNA DNA回收 PCR产物 

分 类 号：Q781[生物学—分子生物学]