叶绿素-a法测定滇池叶绿素-a生长变化案例研究  

Case Study on Growth of Chlorophyll-a in Dianchi Lake by Chlorophyll-a Method

作　　者：段刚[1] 刘晓海[1] 宁平[2] 高云涛[3] 杜刚[3] 铁金刚[3] 

机构地区：[1]云南省环境科学研究院,云南昆明650034 [2]昆明理工大学,云南昆明650093 [3]云南民族大学,云南昆明650031

出　　处：《昆明理工大学学报（理工版）》2006年第3期61-63,共3页Journal of Kunming University of Science and Technology(Natural Science Edition)

基　　金：国家重点基础研究发展计划项目(项目编号:2002CB412310)

摘　　要：在滇池水域中取藻样，过滤、接种在MDM培养基中，置于光照温室中培养，提取出叶绿素-a，然后测定在不同波长下的吸光度值，用叶绿素-a法计算公式计算出叶绿素-a含量．确定适宜的波长范围是用叶绿素-a法测定滇池叶绿素-a生长变化的关键．实验研究结果表明，滇池藻类的叶绿素-a吸收光谱有两个最强的收区：一个是在波长为400～460nm的蓝紫光部分，另一个是在波长为640～660nm的红光部分．在这两个波段下，可测定Fe^2＋对滇池叶绿素-a生长的影响情况。The water samples are taken from Dianchi Lake. After filtered, it is inoculated in MDM, then it is put in glasshouse, the chlorophyll-a of algae is abstracted, the absorbance values of chlorophyll a are measured at the various wavelength, the content of chlorophyll a could be calculated with a formula. Therefore, to determine the suitable scope of wavelength in chlorophyll-a method was a key factor. The experimental results show that absorbance values are strong in the wavelength scope of 400 ～ 460 nm and 640 ～ 660 nm. The case study is carried out by adding Fe^2＋ into experiment sample for measurement effects of Fe^2＋ on ehlorophyll-a in Dianchi Lake under these two wavelength scope.

关 键 词：叶绿素-A 波长 吸光度 藻类 滇池 

分 类 号：X501[环境科学与工程—环境工程]