一氧化氮对肿瘤细胞辐射敏感性的影响研究  

Effects of NO on tumour cells radiosensitivity to γ-rays in vitro

作　　者：孔肇路[1] 金一尊[1] Chinje E C Stratford I J 

机构地区：[1]复旦大学放射医学研究所,上海200032 [2]英国曼彻斯特药学院

出　　处：《辐射研究与辐射工艺学报》2006年第3期171-174,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30500143);国家863计划(2002AA2Z3104)资助

摘　　要：调节肿瘤细胞中一氧化氮(Nitrogenmonoxide,NO)的合成量,观察NO对细胞辐射敏感性的影响。结果显示乏氧和L-甲基盐酸精氨酸(L-NG-Monomethylargininehydrochloride,L-NMMA)作用均可显著抑制NO的合成,但对一氧化氮合酶(Induciblenitrogenmonoxidesynthase,iNOS)活性无显著影响;NO可以增强肿瘤细胞对射线的敏感性也增强,但两者变化并非线性相关;乏氧和L-NMMA作用抑制肿瘤细胞中NO的合成,会降低细胞对射线的敏感性;同母本细胞相比,基因转染建立NO含量较高的肿瘤细胞,氧增比(OxygenEnhancementRatio,ORE)下降,提示NO参与射线引起的细胞损伤。To examine the effect of nitric oxide （NO） on tumour cells radiosensitivity, human sarcoma cells （HT1080） and its inducible nitrogen monoxide synthase （iNOS） gene transfected clones （iNOS9, iNOS12） were exposed to 1-5Gy ^60Co γ-ray irradiation alone or combined with 500μmol/L L-NMMA, a NO synthesis inhibitor, in air or under hypoxia. It showed that both hypoxia and L-NMMA decreased the production of NO, but had no obvious effect on iNOS activity. With more NO formation, gene-tranfered cells showed higher sensitivity to irradiation and lower OER than HT1080, but there was no obvious correlation between NO and cells sensitivity to irradiation. The results indicated that NO could take part in irradiation-induced cells damage and enhance cells radiosensitivity.

关 键 词：一氧化氮 一氧化氮合酶 L-甲基盐酸精氨酸 基因转染 辐射敏感性 

分 类 号：R730.53[医药卫生—肿瘤] R730.55[医药卫生—临床医学]