提取琼胶糖的树脂新方法  

A new method for extracting agarose using basic resin

作　　者：张锐[1] 孙美榕 

机构地区：[1]广东海洋大学现代生化中心,广东湛江524088 [2]深圳市天泰生物技术公司,广东深圳518101

出　　处：《中国海洋药物》2006年第3期28-32,共5页Chinese Journal of Marine Drugs

摘　　要：目的研究一种低成本制备琼胶糖的简便方法。方法以江蓠琼胶粉为原料,采用NH4+型阳离子交换树脂脱除1.5%琼胶溶液中的金属离子,再用OH-型强碱性阴离子交换树脂在恒温70℃下吸附沉降琼胶酯,提取出白色琼胶糖。结果测定的灰分和硫酸根含量均符合电泳琼胶糖的指标,凝胶强度达到560g/cm2,提取率达57%,测定了其结晶紫电泳和DNA的琼胶糖凝胶电泳。结论本方法提取的琼胶糖具有良好的电泳性能,适合用于生物化学和分子生物学的凝胶电泳研究。本法与EDTA-2Na法和DEAE纤维素法相比,是一种低成本制备琼胶糖的简便方法。Objective To study a new method for extracting agarose. Methods In 1.5% agar solution, adding the cation resin exchanged by NH4^＋ to take off metal ions, then using the OH basic resin to absorb agaropectin. Results and Conclusion The extracting rate was 57%, gel strength reached 560g/cm^2 , the agarose has good electricity function by measuring the gentian violet electrophoresis and the DNA gel electrophoresis, so it is suitable for biochemistry and molecular biology electrophoresis. It is a kind of simple and low cost method for extracting agarose which better than the EDTA-2Na method and DEAE cellulose method.

关 键 词：琼胶糖 强碱性树脂 凝胶强度 结晶紫电泳 DNA凝胶电泳 

分 类 号：R931.711[医药卫生—生药学] TQ460.6[医药卫生—药学]