不同辐照度下高浓度锰对黄瓜叶片活性氧产生和抗氧化酶活性的影响  

Effects of High Concentration Manganese on Active Oxygen Production and Antioxidant Enzyme Activities in Cucumber Leaves under Different Irradiations

作　　者：史庆华[1] 朱祝军[1] 王永传[1] 王萍[1] 王秀峰[2] 

机构地区：[1]浙江大学园艺系农业部园艺植物生长发育与生物技术重点开放实验室,杭州310029 [2]山东农业大学园艺科学与工程学院,泰安271018

出　　处：《植物生理与分子生物学学报》2006年第3期325-329,共5页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金重点项目(No.30230250)资助。~~

摘　　要：高浓度锰处理提高了黄瓜叶片中H2O2含量和O-2·的产生速率,导致膜脂过氧化;与自然辐照度相比,1/2辐照度使高浓度锰胁迫下黄瓜叶片活性氧和丙二醛(MDA)的积累均显著降低。高浓度锰处理导致细胞溶质和叶绿体中过氧化氢酶(CAT)活性降低,而其他抗氧化酶的活性升高,尤其是自然辐照度下叶绿体中抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)和谷胱甘肽还原酶(GR)活性的提高幅度比1/2辐照度下更大。高浓度锰处理使自然辐照度下线粒体中抗氧化酶的活性明显升高,而在1/2辐照度下该处理对该酶活性没有显著影响。Effects of high concentration manganese on active oxygen production and antioxidant enzymes in cucumber leaves under natural irradiation and 1/2 natural irradiation were studied. High concentration manganese increased the H202 content and O2^-· producing rate of cucumber leaves （Fig. 1 A, B） and caused lipid peroxidation （Fig. 1C）. Compare to natural irradiation, 1/2 natural irradiation significantly decreased active oxygen produc- tion and lipid peroxidation （Fig. 1）. CAT activities in cytosols and chloroplasts were inhibited by high concentration manganese （Fig.2B）, and other antioxidant enzyme activities were enhanced by high concentration manganese （Fig.2A, C-F）. Especially activities ofAPX, DHAR and GR in chloroplasts were greatly stimulated by high concentration manganese under natural irradiation compared to 1/2 irradiation, which may play important roles in scavenging active oxygen species. Antioxidant enzymes in mitochondria showed higher activities under high concentration manganese under natural irradiation, but the differences were not significant under 1/2 natural irradiation （Fig.2A, C-F）.

关 键 词：黄瓜 高浓度锰 辐照度 亚细胞部分 抗氧化酶 

分 类 号：X173[环境科学与工程—环境科学]