兔脑梗死急性期血浆内皮素及降钙素基因相关肽变化及东菱克栓酶的影响(英文)  

Changes of plasma endothelin and calcitonin gene related peptide and effect of batroxobin in rabbits with acute cerebral infarction

作　　者：杜智勇[1] 黄翠瑶[1] 王钢[1] 

机构地区：[1]解放军广州军区总医院老年病四科,广东省广州市510010

出　　处：《中国临床康复》2006年第28期186-187,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:内皮素和降钙素基因相关肽的动态平衡在脑梗死发生发展的过程中有非常重要的作用和意义。目的:观察东菱克栓酶溶栓治疗对脑梗死后血浆内皮素及降钙素基因相关肽水平的影响。设计:随机对照实验。单位:解放军广州军区总医院老年病四科。材料:实验于解放军广州军区总医院老年病四科完成。选择普通级新西兰老龄兔15只,兔龄18-20个月,平均体质量(3.5±0.5)kg,雌雄不拘。方法:将15只兔子通过改良自体血栓法制备兔脑梗死模型,选模中死亡1只,余14只随机分为对照组和治疗组。治疗组于栓塞后2h耳缘静脉缓慢注入东菱克栓酶,对照组缓慢注入等量生理盐水。观察比较治疗组和对照组脑梗死前后血浆内皮素及降钙素基因相关肽浓度的变化。主要观察指标:术前1h,术后2h(注药前),术后6h(注药后4h)的内皮素及降钙素基因相关肽含量的变化。结果:纳入兔子15只,选模中死亡1只,其余14只兔子进入结果分析。治疗组和对照组兔急性脑梗死术后2h内皮素明显上升(P<0.05,P<0.01),术后6h出现回调下降(P<0.01,P<0.01),其中治疗组下降幅度大于对照组(P<0.01);两组急性脑梗死术后2h降钙素基因相关肽明显下降,术后6h出现回升,治疗组回升幅度大于对照组,但差异无统计学意义(P>0.05)。结论:脑梗死急性期机体对内皮素和降钙素基因相关肽的平衡失调存在自身调节机制,早期使用东菱克栓酶溶栓治疗可使内皮素回调幅度明显增加,这可能是其对脑缺血再灌注损伤的保护机制之一。BACKGROUND： Dynamic balance of endothelin （ET） and calcitonin gene-related peptide （CGRP） plays an important role in occurrence and development of cerebral infarction. OBJECTIVE： To observe the effects of thrombolytic therapy with batroxobin（DF-521） on the levels of plasma ET and CGRP after cerebral infarction. DESIGN： Randomized controlled study. SETTING： Fourth Department of Gereology, General Hospital of Guangzhou Military Area Command of Chinese PLA. MATERIALS： The experiment was performed at the Fourth Department of Gereology, General Hospital of Guangzhou Military Area Command of Chinese PLA. Totally 15 New Zealand rabbits aged from 18-20 months, of common grade and either sex, with the mean body mass of （3.5±0.5） kg were selected. METHODS： A total of 15 rabbits were established into cerebral infarction models with modified autogeneic thrombus method. One rat died when selecting models. Other 14 rats were randomly assigned into control group and treatment group. DF-521 was slowly injected into vein of ear edge of rats in the treatment group at hour 2 after embolism. Saline of the same volume was slowly injected into rats of the control group. Changes of concentrations of plasma ET and CGRP in the treatment group and control group were observed before and after cerebral infarction. MAIN OUTCOME MEASURES： Changes of ET and CGRP contents 1 hour before operation, 2 hours after operation （before drug injection） and 6 hours after operation （4 hours after drug injection）. RESULTS： Of the 15 included rabbits, 1 rat died when establishing model, so 14 rabbits were involved in the result analysis. ET level significantly increased at hour 2 after acute cerebral infarction operation in the treatment group and control group （P 〈 0.05, P 〈 0.01）, and then decreased at hour 6 after operation （P 〈 0.01 ,P 〈 0.01 ）. The decrease in the treatment group was larger than that in the control group （P 〈 0.01 ）. The level of CGRP markedly reduced at hour 2

关 键 词：脑梗死 内皮缩血管肽类 降钙素基因相关肽 安克洛酶 

分 类 号：R743.1[医药卫生—神经病学与精神病学]