人肺腺癌细胞系SPC-A1 mRNA修饰的树突状细胞体外抗肿瘤免疫反应  

Experimental study of antitumor immunity induced by dendritic cell vaccine transfected with tumor cell SPC-A1 mRNA in vitro

作　　者：谢云青[1] 郑秋红[1] 郑天荣[1] 陈蓉明[1] 龚福生[1] 汪相如[1] 

机构地区：[1]福建医科大学教学医院福建省肿瘤医院肿瘤研究所肿瘤分子生物学研究室,福建福州350014

出　　处：《中华肿瘤防治杂志》2006年第11期817-820,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：福建省自然科学基金资助项目(F00019)

摘　　要：目的探讨人肺腺癌细胞系SPC-A1mRNA转染的人外周血树突细胞(dendriticcell,DC)疫苗在体外诱导特异性抗肿瘤免疫反应的能力。方法从健康人外周血分离外周血单个核细胞(peripheralbloodmononuclearcells,PBMC),在白细胞介素-4(interleu-kin-4,IL-4)和人粒细胞集落刺激因子(granulocyte-macrophagecolony-stimulatingfactor,GM-CSF)作用下诱导培养成DC。体外扩增、转录的方法获得人肺腺癌细胞系SPC-A1mRNA,并利用脂质体转染的方法导入DC,流式检测转染后DC表面标记,并与淋巴细胞共孵育,激活T淋巴细胞,MTT法检测其对人肺腺癌细胞的特异性杀伤活性。结果转染后的DC特异性表面标志及功能相关分子表达显著升高(CD8680·32±1·60;HLA-DR86·03±2·58;CD8326·97±1·62),诱导的特异性杀伤反应能力与经肿瘤细胞裂解物负载的DC、未经转染的DC及空白淋巴细胞组相比显著提高(80·34±2·71,P<0·01)。结论人肺腺癌细胞系SPC-A1mRNA转染的DC疫苗体外诱导的特异性抗肿瘤免疫能力显著增强。OBJECTIVE： To study the specific immunoresponse effect of dendritic cells transfected with tumor cells SPC-A1 mRNA. METHODS： DC was got from peripheral blood mononuclear cells （PBMC） induced by granulocyte-macrophage colony-stimulating factor （GM-CSF）, interleukin-4 （IL-4）, and transfected by liposome with tumor cell SPC-A1 mRNA sythesised and amplified in vitro. Surface markers on DC were detected by flow cytometry, co-cultured with unpurifled T cells to generate potent CTL. The cytolytic of specific CTL against SPC-A1 tumor cell was measured by MTT. RESULTS： Surface markers on transfected DC were expressed with significantly higher levels （CD86： 80.32±1.60, HLA-DR： 86.03±2.58; CD83： 26.97±1.62）. Transfected DCs were more potent in stimulating T cell proliferation. Specific CTLs induced by mRNA-transfected DCs showed higher cytotoxicity than non-transfected DCs and tumor cell lysate-pulsed DCs （80.34±2.71, P〈0.01）. CONCLUSION： DCs transfected with tumor cell mRNA are effective vaccines in stimulating specific antitumor T-cell immunity in vitro.

关 键 词：树突细胞 肺肿瘤 RNA 信使 免疫 

分 类 号：R734.2[医药卫生—肿瘤]