全反式维甲酸对前列腺癌PC-3细胞Bcl-2、Bax表达的影响  

Effects of All-trans-retinoic Acid on the Expression of Bcl-2 and Bax in Human Prostate Cancer PC-3 Cell Line

作　　者：王恒兵[1] 严春寅[1] 陈卫国[1] 

机构地区：[1]苏州大学附属第一医院泌尿外科,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2006年第3期447-449,463,共4页Suzhou University Journal of Medical Science

基　　金：江苏省科技发展计划项目资助(No.BS2003013)

摘　　要：目的 探讨全反式维甲酸（ATRA）对人雄激素非依赖性前列腺癌细胞株PC-3增殖的抑制作用及其Bel-2和Bax表达的影响。方法以不同浓度的ATRA处理PC-3细胞，甲基偶氮唑蓝（MTT）比色法检测细胞增殖抑制情况；流式细胞仪和免疫细胞化学法检测PC-3细胞Bax和Bcl-2的表达。结果ATRA浓度为（10^-6～10^-4）mol／L时，可明显抑制PC-3细胞的增殖（P〈0．01），并具有时间-剂量依赖性。ATRA处理后PC-3细胞Bcl-2表达下调，Bax表达上调。随ATRA浓度增加，Bcl-2／Bax的比值不同程度地下降（P〈0．05）。结论ATRA对PC-3细胞具有明显的增殖抑制作用，并且与ATRA下调Bcl-2表达、上调Bax表达有关。Objective To investigate the effects of all-trans-retinoic acid（ATRA） on the proliferation and the expression of Bcl-2 and Bax in human androgen-independent prostate cancer PC-3 cell line. Methods Human prostate cancer PC-3 cell line was cultured in medium containing in different of ATRA concentrations. Cell proliferating inhibition was tested by MTT assay. Flow cytometry and immunocytochemistry were used to analyze the expression of Bcl-2 and Bax in PC-3 cells. Results ATRA inhibited PC-3 cell proliferation in a dose-dependent and time-dependent manner（ P 〈 0.01）. ATRA down-regulated the expression of Bcl-2 and up-regulated the expression of Bax in PC-3 cells. With the increase of ATRA concentration, the ratio of Bcl-2 to Bax decreased gradually. Conclusion ATRA can significantly inhibit the proliferation of PC-3 cells. And it is related with down-regulation of the expression of Bcl-2 and up-regulation of the expression of Bax .

关 键 词：全反式维甲酸 前列腺癌 细胞凋亡 BCL-2 BAX 

分 类 号：R737.25[医药卫生—肿瘤]