凋亡抑制因子在非小细胞肺癌中的表达及其意义  

Expression of inhibitors of apoptosis (IAPs) and its clinical significance in patients with non-small cell lung cancer

作　　者：郎旭宇[1] 冯晋潞[2] 冯珍 赵建滨[4] 解军[4] 

机构地区：[1]山西煤炭中心医院心胸外科,太原030006 [2]山西省肿瘤医院麻醉科 [3]长治市中医院痔科 [4]山西医科大学生物化学与分子生物学教研室

出　　处：《肿瘤研究与临床》2006年第6期370-373,共4页Cancer Research and Clinic

摘　　要：目的探讨凋亡抑制基因(IAPs)家族在非小细胞肺癌(NSCLC)患者组织中的表达及其意义。方法采用半定量反转录聚合酶链反应(RT-PCR)及Western blot分别从mRNA和蛋白水平对36例NSCLC、36例肺部良性病变进行研究,检测生存蛋白(survivin),human IAP-1(hIAP-1),humanIAP-2(hIAP-2)及X chromosome-linked IAP(XIAP)表达情况。结果36例NSCLC组织中有32例survivin mRNA表达阳性,而对照组无一例阳性表达;26例癌组织中XIAP mRNA处于高表达,与对照组比较差异有统计学意义(P<0.05);hIAP-1mRNA仅仅在腺癌组织中高表达(P<0.05),具有组织学类型特异性。Western blot显示在survivin mRNA阳性组织中均可检测到survivin蛋白的表达;XIAP和hIAP-1mRNA高表达的组织同样可检测到相应蛋白。相关性分析表明只有hIAP-1的表达与NSCLC组织学类型相关。结论survivin mRNA高表达可以作为NSCLC辅助诊断的重要指标,hIAP-1可用来协助鉴定NSCLC亚型,survivin和XIAP在肿瘤基因治疗中具有潜在的应用价值和前景。Objective To investigate the expression of inhibitors of apoptosis （IAPs） in non-small cell lung cancer （NSCLC） patients and its clinical significance. Methods The mRNA expression level and protein level of survivin, hIAP-1 （human IAP-1）, hIAP-2 （human IAP-2）, and XIAP （X chromosome-linked IAP） in 36 NSCLC patients and 36 controls wereanalyzed by semi-quantltative reverse transcription polymerase chain reaction （RT-PCR） and Western blotting, respectively. Results High survivin mRNA expression were detected in 32 out of 36 patients, but not in controls. 26/36 NSCLC showed high XIAP mRNA expression and were significantly higher than those with benign diseases （P 〈 0.05）. An elevated hIAP-1 mRNA expression was detected especially in patients with adenocarcinoma （P 〈 0.05）. The tumor specimens with high survivin mRNA expression were also characterized by an elevation in the respective protein level. The enhanced mRNA expression of hIAP-1 and XIAP in respective tumors could also be confirmed on protein level by Western blotting. There was a significant correlation between the expression of hIAP-1 and histopathological type, Conclusion High survivin mRNA expression could be a valuable parameter in NSCLC diagnosis, hIAP-1 might be used to distinguish the subtype of NSCLC. Survivin and XIAP have potentially applied prospect in tumor gene therapy.

关 键 词：凋亡抑制基因 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]