狂犬病病毒糖蛋白、核蛋白融合基因原核质粒的构建与表达  

Construction of Prokaryotic Expression Plasmid with G and N Genes of Rabies Virus and Co-Expression of the Two Genes

作　　者：姜海龙[1] 钱爱东[1] 

机构地区：[1]吉林农业大学动物科技学院,长春130118

出　　处：《吉林农业大学学报》2006年第3期313-316,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(30070542)

摘　　要：试验将编码狂犬病病毒糖蛋白、核蛋白融合基因克隆到原核表达载体pET-32a(+)中,构建狂犬病病毒原核表达质粒。将该质粒转化到大肠杆菌BL21中,经IPTG诱导表达出约2.09×104D的狂犬病病毒糖/核融合基因编码的蛋白质。Western-blotting检测结果表明,该融合蛋白质具有免疫原性。The experiment was conducted to construct pmkaryotic expression plasmid containing the G and N melt genes of rabies virus through cloning the genes of rabies virus into pET-32a（ ＋ ）. After transforming the plasmid into E. coli BL21, the bacteria were induced by IPTG and the protein was detected with western-blotting test. The result indicated that the two genes could be co-expressed by the pmkaryotic expression plasmid, which will supply foundation for the preparation of the antigen use to diagnose rabies.

关 键 词：狂犬病病毒 糖蛋白 核蛋白 原核表达质粒 共表达 

分 类 号：S852.655[农业科学—基础兽医学]