利用定量杂交法检测绵羊瘤胃纤维细菌的研究  

Use of 16S-rRNA Hybridization Probes to Detect the Cellulolytic Bacteria in Rumen

作　　者：王海荣[1] 侯先志[1] 高爱武[1] 吕莉华[1] 郭媛[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,呼和浩特010018

出　　处：《微生物学通报》2006年第3期7-10,共4页Microbiology China

基　　金：国家自然科学基金资助项目(No.30460095)

摘　　要：实验利用通用细菌探针和3株纤维分解菌的特异性探针,初步建立起对瘤胃细菌进行检测的16S rRNA定量杂交的方法。试验将提取的总RNA按浓度系列稀释后与通用细菌探针进行杂交,检测结果所做的回归分析表明,杂交信号与尼龙膜上的所点RNA的量具有明显线性关系。同时对几份瘤胃样品进行3种纤维分解菌的初步定量检测,结果显示3种纤维分解菌的相对丰度与前人报道相似,表明该方法能够对瘤胃细菌进行定量分析,可在后续相关研究中使用。In this study, the general bacterial probe and specific cellulolyfie bacterial probes were used to quantify the bacteria in rumen. The total RNA were extracted and then hybridized with general bacterial probe after a dilution of concentration. The result showed that there was a high correlation between the hybridization signal and the dilution of total bacterial RNA. Based on the result above, the quantities of throe cellulolytic bacteria in rumen sample were detected. The comparative RNA percentage of three cellulolytic bacteria to total bacterial RNA were similar to the previous reports. It can be concluded that the quantification of bacteria in rumen could be condacted by this approach, and which could be used in future research.

关 键 词：16S RRNA 寡核苷酸探针 定量杂交 瘤胃细菌 

分 类 号：S852.6[农业科学—基础兽医学]