Synchrotron Small-Angle X-Ray Scattering Investigation on Integral Membrane Protein Light-Harvesting Complex LH2 from Photosynthetic Bacterium Rhodopseudomonas Acidophila  

作　　者：都鲁超 翁羽翔 洪新国 冼鼎昌 Kobayashi Katsumi 

机构地区：[1]Laboratory of Soft Matter Physics, and Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080 [2]Beijing Synchrotron Radiation Facility (BSRF), Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049 [3]Photon Factory, High Energy Accelerator Research Organization 1-10ho, Tsukuba-shi, Ibaraki-ken, 305-0801, Japan

出　　处：《Chinese Physics Letters》2006年第7期1861-1863,共3页中国物理快报（英文版）

基　　金：Supported by the National Natural Science Foundation of China under Grant Nos 10375075, 60321003, and 60438020, the National Key Basic Research and Development Programme of China under Grant No G1998010102, and the Knowledge Innovation Project of Chinese Academy of Sciences under Grant No KJCX2-SW-W14.

摘　　要：Structures of membrane protein in solution are different from that in crystal phase. We present the primary results of small angle x-ray scattering （SAXS） resolved topological structures of a light harvesting antenna membrane protein complex LH2 from photosynthetic bacteria Rhodopseudomonas acidophila in detergent solution for the first time. Our results show that the elliptical shape of the LH2 complex in solution clearly deviates from its circular structure in crystal phase determined by x-ray diffraction. This result provides an insight into the structure and function interplay in LH2.Structures of membrane protein in solution are different from that in crystal phase. We present the primary results of small angle x-ray scattering （SAXS） resolved topological structures of a light harvesting antenna membrane protein complex LH2 from photosynthetic bacteria Rhodopseudomonas acidophila in detergent solution for the first time. Our results show that the elliptical shape of the LH2 complex in solution clearly deviates from its circular structure in crystal phase determined by x-ray diffraction. This result provides an insight into the structure and function interplay in LH2.

关 键 词：HIGH-RESOLUTION B850 BAND SPECTROSCOPY SHAPE RHODOPSIN 

分 类 号：TL5[核科学技术—核技术及应用]