Effects of Matrix-to-analyte Ratio and Laser Energy on Peptides Ion Signals  

作　　者：Liu-zhu Zhou Yuan Zhu Xiao-yong Guo Wen-wu Zhao Hai-yang Zheng Xue-jun Gu Li Fang Wei-jun Zhang 

机构地区：[1]Lab of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China [2]Qufu Normal University, Qufu 273165, China

出　　处：《Chinese Journal of Chemical Physics》2006年第3期207-210,共4页化学物理学报（英文）

摘　　要：A method of aerosol introduction for matrix-assisted laser desorption/ionization （MALDI） is described. The aerosol particles containing matrix and analyte enter directly into the aerosol time-of-flight mass spectrometer （ATOFMS） at atmospheric pressure. The scattered light signals from the aerosol particles are collected by a photomultiplier tube （PMT） and are passed on to an external electronic timing circuit, which determines particle size and is used to trigger a 266 nm pulsed Nd：YAG laser. The aerosol MALDI mass spectra and aerodynamic diameter of single particles can be obtained in real-time. Compared with other methods of liquid sample introduction, this method realizes detection of single particles and, more importantly, the sample consumption is lower. The effects of matrix-to-analyte ratio and laser pulse energy on analyte ion yield are examined. The optimal matrix-to-analyte ratio and laser energy are 50-110：1 and 200-400μl respectively.

关 键 词：ATOFMS MALDI MATRIX PEPTIDES 

分 类 号：O657.63[理学—分析化学]