激光显微捕获口腔上皮细胞的DNA分型  

DNA Genotyping of Oral Epithelial Cells by Laser Capure Microdissection

作　　者：顾丽华 张晨 陈连康 郑会芬 程莉 周怀谷 

机构地区：[1]上海市刑事科学技术研究所,上海200083

出　　处：《法医学杂志》2006年第3期196-197,203,共3页Journal of Forensic Medicine

摘　　要：目的探索激光显微技术(lasercapturemicrodissectionsystem,LCM)捕获口腔上皮细胞,并进行STR-DNA分型检测的方法。方法用VERITAS显微切割仪红外低能激光显微捕获一定数量口腔上皮细胞,进行ProfilerPlus试剂盒STR复合扩增,检测DNA基因型。结果7～8个口腔上皮细胞能成功获得STR-DNA分型。3～4个口腔上皮细胞不能成功获得STR-DNA分型。结论激光显微捕获作为一种分离单个细胞的新技术,对于微量口腔上皮细胞的STR-DNA分型是可行的。objective The STR genotypping of trace oral epithelial cells which are microdissected by laser capure microdissection system （LCM） is explored. Methods The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures. Results DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed. Conclusion It is viable in genotyping of trace oral epithelial cells by Laser Capure Microdissection as a new technology of seperating single cell.

关 键 词：激光显微捕获(LCM) 口腔上皮细胞 STR-DNA分型 

分 类 号：R781.5[医药卫生—口腔医学] R392.2[医药卫生—临床医学]