曲安奈德对高糖培养人视网膜色素上皮细胞细胞间黏附分子-1表达的影响  

Effect of triamcinolone acetonide on expression of intercelluar adhesion molecule -1 of high glucose cultured human retinal pigment epithelial cells

作　　者：韩小霞[1] 惠延年[1] 宋虎平[1] 王海涛[2] 张自峰[1] 

机构地区：[1]第四军医大学西京医院眼科全军眼科研究所,中国陕西省西安市710032 [2]第四军医大学西京医院血液科,中国陕西省西安市710032

出　　处：《国际眼科杂志》2006年第3期596-598,共3页International Eye Science

摘　　要：目的:观察曲安奈德(TA)对高浓度葡萄糖条件下体外培养人视网膜色素上皮(hRPE)细胞细胞间黏附分子-1(I-CAM-1)表达的影响。方法:用MTT方法检测不同浓度(0.01,0.10和1.00g/L)的TA对hRPE细胞增生的影响,用免疫荧光法检测加用和未加用0.10g/LTA的30.0mmol/L葡萄糖作用1,2和3d后hRPE细胞表面ICAM-1的表达强度。结果:在0.01～1.00g/L浓度TA的作用下,hRPE细胞生长受到抑制,呈剂量依赖性和时间依从性。加用0.10g/LTA的30.0mmol/L葡萄糖作用1,2和3d后,hRPE细胞表面荧光染色密度分别比未加用TA的30.0mmol/L葡萄糖作用下荧光着色减弱20%,25%和50%,有统计学显著性差异(P<0.05)。结论:TA可以抑制体外高浓度葡萄糖培养的hRPE细胞的增生,也抑制高糖作用下hRPE细胞表面的ICAM-1表达。AIM： To investigate the effects of triamcinolone acetonide （TA）on the expression of intercelluar adhesion molecule-1 （ I- CAM-I） of human retinal pigment epithelial （hRPE） cells cultured with high glucose in vitro. METHODS： The effect of TA at various concentrations （0.01, 0.10 or 1.00g/L） on the proliferation of hRPE cells was detected by methyl thiazolyl tetrazolial （MTr） assay. The expression of ICAM-1 of hRPE cells cultured with 30.0mmol/L of glucose with or without 0.1g/L of TA for 1, 2, and 3d was measured with immunofluorescence staining. RESULTS： At the concentrations of 0.01, 0.10, or 1.00g/L of TA inhibited the growth of hRPE cells in a dose-dependent manner （P〈0.05, compared with the control）, and the fluorescence staining density on the cell membrane of hRPE cells cultured with 0.1g/L of TA and with 30.0mmol/L of glucose for 1, 2 and 3d was decreased by 20%, 25%, and 50% of that without TA in 30.0mmol/L of glucose, respectively. The difference between the two groups was statistically significant （P〈0.05）. CONCLUSION： TA can inhibit the growth of hRPE cells in vitro with high glucose, hRPE cells cultured with high glucose can enhance expression of ICAM-1 of the cells and that can be inhibited by TA. It is suggested that TA may interrupt pathological reaction of diabetic retinopathy by inhibiting expression of ICAM-I of hRPE cells.

关 键 词：人视网膜色素上皮细胞 高浓度葡萄糖糖 细胞间黏附分子-1 曲安奈德 

分 类 号：R774.1[医药卫生—眼科]