纤维连接蛋白基因EDA片段在腺样囊性癌细胞中的表达  

The Expression of EDA Region of Fibronectin in Salivary Adenoid Cystic Carcinoma Cells

作　　者：张建鹏[1] 李翠英[1] 

机构地区：[1]首都医科大学附属北京口腔医院

出　　处：《首都医科大学学报》2006年第3期290-293,共4页Journal of Capital Medical University

基　　金：国家自然科学基金(30371547)资助项目

摘　　要：目的通过纤维连接蛋白(FN)基因EDA片段在不同转移潜能腺样囊性癌(SACC)细胞中的表达,研究EDA片段与SACC细胞生物学特性的关系。方法应用免疫组织化学及半定量反转录聚合酶链反应(RT-PCR)法,分别从蛋白水平及mRNA水平对高、低转移SACC细胞株中FN基因EDA片断进行检测。结果高转移SACC细胞株(SACC-LM)中FN基因EDA片段蛋白及mRNA的表达高于低转移细胞株(SACC-83)。结论FN基因EDA片段的表达程度与SACC细胞不同转移潜能密切相关。Objective To investigate the expression of EDA region of fibronectin（FN） in salivary adenoid cystic carcinoma （SACC） cells and deduce the relationship between EDA and SACC biological characteristic. Methods Immunohistochemistry and reverse transcriptase polymerase chain reaction（RT-PCR） techniques were used to detect EDA region proteins and mRNAs in different metastatic potential SACC cells. Results The expression of EDA region proteins and mRNAs in high metastatic SACCLM cells were higher than those in low metastatic SACC-83 cells. Conclusion The expression of EDA region of FN may play an important role in the invasion and metastasis of SACC.

关 键 词：腺样囊性癌 纤维连接蛋白 EDA片段 转移 

分 类 号：R739.81[医药卫生—肿瘤]