Nordy对人腺泡型横纹肌肉瘤细胞系PLA-802的诱导分化作用  

Nordy induces differentiation of human alveolar rhabdomyosarcoma cell line PLA-802 and its xenografts in mice

作　　者：刘斌[1] 米粲[1] 卞修武[2] 陈代伦[2] 

机构地区：[1]重庆医科大学基础医学院病理学教研室,重庆400016 [2]第三军医大学附属西南医院病理研究所,重庆400038

出　　处：《临床与实验病理学杂志》2006年第3期336-339,343,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家863计划引导项目(2002AA001010)

摘　　要：目的观察Nordy对人腺泡型横纹肌肉瘤细胞系PLA-802细胞的体内和体外诱导分化作用。方法用终浓度100μmol/L Nordy处理体外培养细胞,于处理后第24、48、72和96h时相点用倒置显微镜观察细胞形态变化;右腋皮下接种了PLA-802细胞的裸鼠于接种后第7天开始按27mg/kg Nordy隔日1次经腹腔注射给药,共给药10次,于接种后第26天杀鼠摘取移植瘤。细胞和移植瘤组织经HE、电镜和myosin免疫组化染色,光镜、电镜观察及免疫组化图像分析。结果用Nordy处理体内外生长的PLA-802细胞后,瘤细胞核质比降低;细胞形态变椭圆形或梭形,核变小呈椭圆形,核仁变小,核分裂象减少;免疫组化显示胞质内myosin蛋白表达增强;电镜见胞质中肌(微)丝明显增多,甚至有横纹肌肌原纤维形成。结论Nordy对体内外生长的PLA-802细胞具有明显的诱导分化作用。Purpose To investigate the effects of Nordy on differentiation of human alveolar rhabdomyosarcoma cell line PLA-802 in vitro and in vivo. Methods PLA-802 cells of were treated with 100 μmol/L Nordy in vitro, changes of cell morphology examined and cell specimen collected at the time points of 24th, 48th, 72th and 96th hours after treated with Nordy. Nude mices that had been implanted PLA-802 by s, c. injection into the right armpit before 7 days were given 10 times i, p, of 27 mg/kg Nordy, once every two days. Animals were euthanized on the 26th day after tumor cells transplantation for getting the xenografts. The cells and xenograft tissues were stained of HE, EM and myosin immunostainning, and then examined with light microscopy, electron microscopy or image analysis by computer. Results After treatment PLA-802 with Nordy in vitro and in vivo, nuclei-cytoplasmic ratio of tumor cells was decreased, shapes of neoplastic cell were changed into oval or fusiform, and nuclei into oval with decreased size. Nucleolus was smaller in size and karyokinesis was less than untreatment. There was increased myosin protein expression stained by myosin immunostainning and large amounts of myofilament, even muscle fibril in cytoplasm viewed by electron microscopy. Conclusions Nordy can significantly induce differentiation of human alveolar rhabdomyosarcoma cell line PLA 802 in vitro and in vivo.

关 键 词：横纹肌肉瘤 NORDY 细胞系 肿瘤诱导分化 

分 类 号：R738.7[医药卫生—肿瘤]