去甲斑蝥素微球对兔肝动脉栓塞作用的研究  

Effect of norcatharidin microspheres on embolization of hepatic artery in rabbits

作　　者：李琦[1] 范忠泽[1] 孙珏[1] 李先茜[1] 刘晓华[2] Paul Heng 张闽光[3] 

机构地区：[1]上海中医药大学附属普陀医院肿瘤科,200062 [2]新加坡国立大学药学系 [3]上海中医药大学附属曙光医院放射科

出　　处：《介入放射学杂志》2006年第6期360-363,共4页Journal of Interventional Radiology

基　　金：上海市自然科学基金资助项目(03ZR14083);上海市卫生局青年基金资助项目(034y41);中新(新加坡)国际合作项目(NUS-SPT200402)

摘　　要：目的观察去甲斑蝥素鄄海藻酸/聚酸酐微球(NAPMS)对兔肝动脉的栓塞作用。方法新西兰兔18只,在DSA下,行肝动脉造影后,以8mg/kg的剂量经肝动脉注入NAPMS,注入后10min、1,7、14、21和30d各取3只再次造影,观察肝动脉栓塞情况,并处死,取心、肝、肾、脾、肺、胰、胃等组织,观察病理变化,同时作肝、肾功能、血常规检查。结果兔肝动脉栓塞前,肝脏血管造影清晰,栓塞后10min造影,远端微血管消逝,肝动脉增粗、迂曲。介入栓塞后第1、7、14、21和30天造影远端血管均未显影。肝脏病理结果显示微球栓塞于肝窦前小动脉。栓塞后出现一过性肝功能损害,AST,ALT均在栓塞后1d达最高值,以后逐渐下降,7d左右恢复正常水平(P>0.05)。栓塞后白细胞出现一过性升高,第3天达最高值,第7天接近正常水平(P>0.05)。结论去甲斑蝥素微球具有良好的肝动脉末梢栓塞作用,栓塞时间在1个月以上,是较理想的介入栓塞剂。Objective To observe the effect of norcatharidin-alginic acid-polyacid anhydride microspheres （NAPMS）on embolization of hepatic artery in rabbits. Methods Under digital subtracted angiography （DSA）, hepatic angiographies were performed in 18 New Zealand white ragbbits with injection of norcantharadin microspheres（8 mg/kg）via hepatic artery. At 10 minutes and on the 1 st, 7 th, 14 th, 21 st and 30 th day, three rabbits from each group were taken angiography again to display the hepatic embolization status. After sacrificing these rabbits, the tissues of their hearts, livers, kidneys, spleens, lungs, pancreases and stomachs were obtained for examination of their pathological changes. With simultaneously functional tests of liver and kidney in addition of blood routine. Results Before hepatic embolization, the angiographic images showed clear delineation of hepatic vessels.The disappearance of distal microvessels with thicker and twisted hepatic arteries accurred at 10 min after the emblization. There was no visualization of distal vessels on the 1 st, 7 th, 14 th 21 st and 30 th day after embolization. The pathological analysis revealed that the microspheres were mainly present in presinusoidal arterioles after the embolization. The transient liver function damage occurred after embolization with both AST and ALT reaching the peak levels on the 1st day, which decreased gradually and then returned to normal at 7 th day（P 〉 0.05）. Simultaneously, the transient elevation of white blood cells occurred, approaching the peak level on 3 rd day and back to the normal on 7 th day （P 〉 0.05）. Conclusions NAPMS possesses a better embolitic action on the termina hepatic arteries, lasting for more than one month and providing as an ideal interventional embolizing agent.

关 键 词：介入治疗 去甲斑蝥素 栓塞 微球 

分 类 号：R730.5[医药卫生—肿瘤]