肝癌患者肝癥口服液含药血清对SMMC-7721肝癌细胞TGFβ受体表达的影响  

Effects of drug serum of Ganzheng Solution(肝癥口服液) on TGF beta receptor of SMMC-7721 cell line

作　　者：姚树坤[1] 孔丽[2] 张瑞星[1] 殷飞[1] 李胜棉[1] 王娜[1] 

机构地区：[1]河北医科大学第四医院消化内科,石家庄050011 [2]河北医科大学第三医院中西医结合肝病科,石家庄050051

出　　处：《中药药理与临床》2006年第3期169-171,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河北省自然科学基金资助项目;项目编号:303516

摘　　要：目的:观察肝癥口服液含药人血清对SMMC-7721肝癌细胞TGFβ1/Sm ads信号转导通路中TGFβ受体(TGFβR)的影响。方法:以健康志愿者和肝癌患者用药前后血清培养人SMMC-7721肝癌细胞,应用RT-PCR和W estern B lotting技术观察含药血清对肝癌细胞TGFβRⅠ和TGFβRⅡmRNA和蛋白表达的影响。结果:健康志愿者服用肝癥口服液前后血清对SMMC-7721细胞的TGFβRⅠ和TGFβRⅡ蛋白和mRNA表达无显著影响;肝癌患者肝癥口服液的含药血清可使明显受到抑制的TGFβRⅠ和TGFβRⅡ表达恢复,尤其以TGFβRⅡ表达显著上调。结论:肝癌患者肝癥口服液含药血清可增加肝癌细胞TGFβR的表达,使受到抑制的TGFβ1/Sm ads信号转导通路功能恢复。Objective： To observe the changes of TGFβ1/Smads signaling pathway of SM MC-7721 cells cultured with drug serum of Ganzheng solution. Methods： The SMMC-7721 cells were cultured by serum of hepatocellular carcinoma （HCC） patients or healthy volunteers before and after taken Ganzheng solution. The expression of TGFβR Ⅰ and TGFβR Ⅱ protein and mRNA of SMMC-7721 cells were examined by Western blotting and RT-PCR assay. Results： Ganzheng solution drug serum of HCC patients could enhance the expression of TGFβR Ⅰ and TGFβR Ⅱ protein and mRNA in SMMC-7721 cells, especially TGFβR Ⅱ expression （P 〈 0.05）. Conclusions： Ganzheng solution drug serum of HCC patients could activate the function of TGFβ1/Smads signaling pathway, which might be one of the mechanisms of anticarcinoma of Ganzheng solution.

关 键 词：肝癥口服液 肝癌细胞SMMC-7721 TGFβ1/Smads信号转导通路 TGFΒ受体 含药血清 

分 类 号：R735.7[医药卫生—肿瘤] R736.102[医药卫生—临床医学]