电针“足三里”穴对内脏痛大鼠延髓内脏带内c-fos和GFAP表达的影响  

Effects of Electroacupuncture at “Zusanli” (ST 36) on the Expression of c-fos and Glial Fibrillary Acidic Protein in the Medullary Visceral Zone in Visceral Pain Rats

作　　者：秦明[1] 黄裕新[1] 王景杰[1] 段丽[2] 曹荣[2] 饶志仁[2] 

机构地区：[1]第四军医大学唐都医院消化内科,西安710032 [2]第四军医大学神经科学研究所,西安710038

出　　处：《针刺研究》2006年第3期136-139,148,F0003,共6页Acupuncture Research

基　　金：国家自然科学基金资助项目(No.39970888)

摘　　要：目的：探讨电针对内脏痛大鼠模型疼痛行为学方面的影响以及延髓内脏带中即刻早期基因c-fos和胶质原纤维酸性蛋白（Glial Fibrillary Acidic Protein，GFAP）在针刺镇痛中的表达及其意义。方法：雄性SD大鼠32只，随机分为空白对照组、内脏痛组、电针组和电针后内脏痛组，每组8只。将0．6％乙酸（V／V，10mL／kg）注射入大鼠腹腔内造成内脏痛模型。电针取双侧“足三里”穴，断续波，频率50Hz，强度2～5V，持续30min。观察并计算各组动物的疼痛指数（visceral pain index，VPI）后处死动物，延髓切片进行抗Fos蛋白或抗GFAP单一或双重标记的免疫组织化学染色，观察并记数c—fos和GFAP在中枢延髓内脏带内的表达。结果：内脏痛组和电针后内脏痛组出现典型的扭体反应，其中内脏痛组更明显，而空白对照组和电针组大鼠行为学无变化。各组c-fos阳性神经元、GFAP阳性星形胶质细胞表达主要位于延髓内脏带内孤束核、迷走神经背侧运动核。除了空白对照组，内脏痛组、电针组和电针后内脏痛组中c-fos和GFAP表达明显增高；3组之间比较，c-fos和GFAP表达内脏痛组最高，电针后内脏痛组其次，电针组最低（P〈0．01）。结论：电针“足三里”穴对内脏痛模型大鼠的疼痛具有良性调节作用，其机制可能与其调节延髓内脏带内免疫阳性神经元和星形胶质细胞的功能活动有关。Objective： To observe the effect of electroacupuncture （EA） on visceral pain and investigate the significance of the expression of c-fos and glial fibrillary acidic protein （GFAP） in medullary visceral zone （MVZ） in experimental visceral pain rats. Methods： Thirty-two male SD rats were randomized into control, visceral pain （VP）, EA＋ saline and EA＋ VP groups, with 8 rats in each group. Visceral pain model was established by intraperitoneal injection of 0.6% acetic acid （10 mL/kg）. EA （50 Hz, 2-5 V, intermittent waves） was applied to bilateral ＂Zusanli＂（ST 36） for 30 rain followed by establishment of VP. Saline （10 mL/kg） was given （i. p. ） to the rats after EA. Immuno-histochernical single or double staining methods （ABC） were used to show the immunoactivity of c-fos and GFAP in MVZ including nucleus of solitary tract （NTS） and dorsal motor nucleus of the vagus nerve （dmnx）. The degree of visceral pain was evaluated by visceral pain index （VPI）, according to the Schmauss exponential method. Results. Pain reactions （writhing reactions, abdominal muscular contraction, hind-paw twitching, etc） happened in rats of model group and EA＋VP group after injection of acetic acid, being more serious in the former group, and no such reactions were found in control and EA＋ saline groups. Fos-imrnunoreaction （IR）-positive neurons of VP, EA＋ saline and EA ＋ VP groups found mainly in NTS and dmnx were significantly more than those of control group （P 〈 0.05）, and in comparison with VP group, c-fos-IR-positive neurons of EA ＋ saline and EA ＋ VP groups were significantly fewer （P 〈 0.05）. The optical grey values of GFAP-IR-positive microglial cells found mainly in NTS and dmnx in VP, EA＋ saline and EA＋ VP groups were markedly higher than that of control group （P〈0.05） ; while compared with VP group, the grey values of GFAP activity of EA＋saline and EA＋,VP groups were significantly lower （P 〈 0.05）. Compared with VP gr

关 键 词：内脏痛 针刺镇痛 C-FOS GFAP 延髓内脏带 

分 类 号：R573[医药卫生—消化系统]