小蓟及小蓟炭指纹图谱的初步研究  

Study of Fingerprint Analysis of Herba Cirsii and Herba Cirsii Carbonisatus

作　　者：陈毓[1] 丁安伟[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《上海中医药大学学报》2006年第2期62-65,共4页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家"十五"攻关基金资助项目(2001BA701A55-40)

摘　　要：目的:采用高效液相色谱法建立小蓟、小蓟炭的指纹图谱,并进行分析比较。方法:用KromasilC18(46×250mm,5μm)色谱柱,0.5%冰醋酸溶液—乙腈为流动相,采用梯度洗脱,流速1.0ml/min,检测波长254nm,柱温:30℃。结果:初步建立了小蓟、小蓟炭的指纹图谱,其精密度、重复性、稳定性试验中共有峰相对保留时间和相对峰面积的RSD均小于3%。结论:该方法简便、准确、重复性好,小蓟炒炭前后指纹图谱有明显差异。Objective：To establish the fingerprints of herba Cirsii and Herba Cirsii Carbonisatus by reversed-phase performance liquid chromatography （PR-HPLC） and analyze them. Methods： Chromatographic conditions were available as follows： Kromasil C18 （46×250mm, 5μm）, 0.5% glacial acetic acid solution-methylcyanide as mobile phase, gradient elution, flow rate 1.0 ml/min, the detection wavelength 254 nm, column temperature 30℃. Results：The fingerprints of herba Cirsii and herba Cirsii carbonisatus were basically established. The RSD of relative peak areas and retention time of common peak in precision, repeatability and sta- bility tests were lower than 3.0%. Conclusion： This method is convenient, accurate and repeatable to use as a standard method in quality control of herba Cirsii. Great difference occurred in its fingerprint after the herba Cirsii was carbonized.

关 键 词：小蓟 小蓟炭 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]