肝素-ELISA法检测血清中期因子及其对肝癌诊断的初步应用  

Heparin-enzyme linked immunosorbent assay in determination of serum midkine concentration and its application in diagnosis of hepatocellular carcinoma

作　　者：李瑾[1] 钱海华[1] 康晓燕[1] 杨之斌[1] 吴孟超[1] 殷正丰[1] 

机构地区：[1]第二军医大学东方肝胆外科医院分子肿瘤实验室,上海200438

出　　处：《第二军医大学学报》2006年第6期631-633,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30400531);国家"973"计划项目(2002CB513100)~~

摘　　要：目的：建立中期因子（midkine,MK）的测定方法,初步探讨血清MK浓度测定对肝癌的诊断价值.方法：根据MK可结合肝素的特点,应用肝素-抗体双夹心原理,建立肝素-ELISA,并检测20例健康献血者、20例良性肝占位性病变患者、20例肝硬化患者以及104例肝细胞癌（HCC）患者血清MK的质量浓度.结果：肝素-ELISA检测MK的最小值为15 ng/L,可测范围为15～1 000 ng/L,＜500 ng/L时呈线性关系（r=0.97）.测定结果表明,正常献血者、良性肝占位性病变患者和肝硬化患者血清MK浓度分别为（56±11）、（74±19）和（89±43）ng/L,而HCC患者血清MK浓度为（199±87）ng/L,与前3组之间有显著差异（P＜0.05）.HCC患者血清MK阳性率达83.7%,与其他3组之间具有显著性差异（P＜0.001）.结论：应用肝素-ELISA测定血清MK的质量浓度对肝癌具有诊断价值.Objective： To develop a method for determination of serum midkine （MK） concentration and to evaluate its value in the diagnosis of hepatocellular carcinoma （HCC）. Methods： Since heparin can bind to MK, a heparin-enzyme linked immunosorbent assay （ELISA） for determination of MK was developed using heparin-antibody sandwich technique. Serum MK levels were measured in 20 healthy adults, 20 patients with benign liver tumors, 20 with liver cirrhosis,and 104 with HCC. Results： The method had a minimum detectable concentration of 15 ng/L and a detectable range of （15-1 000） rig/L, and a linearity was found when the concentration was 〈500 ng/L（r=0.97）. Serum MK levels of the healthy subjects,the patients with benign liver tumor, liver cirrhosis and HCC were （56±11） rig/L,（74±19） ng/L, （89±43） ng/L and （199±87） ng/L, respectively; with significant difference found between HCC group and the former 3 groups （P〈0.05）. The positive rate of MK in HCC group was 83.7% ,significantly higher than those of 3 other groups （P〈0. 001）. Conclusion： The heparin-ELISA method for MK determination has diagnostic value for HCC.

关 键 词：肝素 酶联免疫吸附 中期因子 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]