外源DNA导入木豆及其在育种上的应用  

Technological Study on Introduction of Exogenous DNA into Pigeon Pea and Its Application in Breeding

作　　者：易鹏[1,2] 侯开卫[1,2] 周家齐[1,2] 马显达[1,2] 张建云[1,2] 王芳[1,2] 

机构地区：[1]中国林业科学研究院资源昆虫研究所 [2]云南省农业科学院

出　　处：《林业科学研究》1996年第5期530-533,共4页Forest Research

基　　金：云南省科委自然科学基金

摘　　要：外源ＤＮＡ导入木豆及其在育种上的应用＊易鹏侯开卫周家齐马显达张建云王芳关键词木豆、外源ＤＮＡ导入、育种木豆（Ｃａｊａｎｕｓｃａｊａｎ（Ｌ．）Ｍｉｌｓｐ．）系南亚热带分布较广的具有较高经济价值的木本植物。国外对这一树种有专门的研究所进行综合性研究。国内...An experiment has been made on the introduction of general DNA extracted from soy bean into 860 flowers of pigeon pea and 620 different individuals have thus been obtained. Single selection and succession breeding have been carried out and 13 family systems of progeny of the single plants developed with remarkable mutant characteristics. And according to the protein analysis of their seeds with SDS PAGE, IEF,it has been found that five individual plants have shown the presence of a band spectrum of soy bean protein. The seeds of these different plants have been applied to establish D 2 and D 3 generation groups and their germination and survival rate have got up to 95% respectively. Finally,a varied material of pigeon pea with high protein contents has been obtained in the light of analysing the components and contents of the seeds of the plant of the D 3 generation. It′s protein content is 30.88%,which is much more than that of the receptor,pigeon,by 66%. And the protein contents of the other four have reached above 20%. Through breeding for third generation of D 3,it has shown that the features of these varied plants are relatively stable.

关 键 词：木豆 外源DNA导入 育种 

分 类 号：S529.035.3[农业科学—作物学]